PMID- 1657544
OWN - NLM
STAT- MEDLINE
DCOM- 19911211
LR  - 20150901
IS  - 0253-2662 (Print)
IS  - 0253-2662 (Linking)
VI  - 24
IP  - 1
DP  - 1991 Feb
TI  - Detection of human herpesvirus 6 DNA in T and B cell lymphomas.
PG  - 44-54
AB  - DNA extracted from tissues of 109 patients with lymphoproliferative disorders, 
      including 40 T-cell lymphomas, 40 B-cell lymphomas, 8 Hodgkin's disease, 13 
      hyperplasia, 5 infectious mononucleosis, 2 histiocytic medullary reticulosis and 
      1 Kikuchi's disease, and peripheral blood lymphocytes (PBL) from 17 healthy 
      donors were investigated for the presence of human herpesvirus 6 (HHV-6) DNA 
      sequence by polymerase chain reaction (PCR) and Southern hybridization. The 
      procedures for DNA amplification were composed of the first round of PCR using a 
      pair of primers LL and LR, which were contained in the HHV-6 major capsid gene, 
      and the second round of nested PCR using an internal pair of primers LLN and LRN. 
      Results showed that the prevalence of HHV-6 DNA sequence in human tissues was 
      similar in both B and T cell lymphoma patients and normal adults, and viral 
      heterogeneity was detected in the amplified DNA sequence from local strain of 
      Taiwan as compared to that of U1102 strain from Uganda.
FAU - Chen, J Y
AU  - Chen JY
AD  - Graduate Institute of Microbiology, College of Medicine, National Taiwan 
      University, Taipei, Republic of China.
FAU - Cheng, C Y
AU  - Cheng CY
FAU - Hsu, T Y
AU  - Hsu TY
FAU - Su, I J
AU  - Su IJ
FAU - Pao, C C
AU  - Pao CC
FAU - Liu, M Y
AU  - Liu MY
FAU - Yang, C S
AU  - Yang CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China (Republic : 1949- )
TA  - Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi
JT  - Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of 
      microbiology and immunology
JID - 8008067
RN  - 0 (DNA, Viral)
SB  - IM
MH  - DNA, Viral/*analysis
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - Lymphoma, B-Cell/*microbiology
MH  - Lymphoma, T-Cell/*microbiology
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction
MH  - Reference Values
MH  - Sensitivity and Specificity
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1991 Feb;24(1):44-54.