PMID- 16579976
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20200319
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1085
IP  - 1
DP  - 2006 Apr 26
TI  - Somato-dendritic distribution of 5-HT(1A) and 5-HT(1B) autoreceptors in the BDNF- 
      and cAMP-differentiated RN46A serotoninergic raphe cell line.
PG  - 121-6
AB  - The rapid differentiating effects of brain-derived neurotrophic factor (BDNF) or 
      dibutyryl-cAMP (dBcAMP) were characterized on RN46A, a rat raphe-derived neuronal 
      cell line. After BDNF treatment, RN46A cells were serotonin-immunopositive and 
      bipolar, and expressed the microtubule-associated-protein 2 (Map2). After dBcAMP 
      treatment, the cells often became multipolar, bearing very long processes 
      strongly immunopositive for serotonin and Map2. Under both conditions, the 
      expression and distribution of 5-HT(1A) and 5-HT(1B) autoreceptors remained 
      identical. 5-HT(1A) and Map2 immunolabelings were superimposable, as expected of 
      their somato-dendritic targeting. Surprisingly, the distribution of 5-HT(1B) 
      immunoreactivity was similar, in contrast with its usual localization in axons 
      and nerve terminals in the brain. In conclusion, both BDNF and 
      cAMP-differentiated RN46A cells towards a neuronal serotoninergic-like phenotype 
      without the typical differential targeting of the 5-HT(1) autoreceptors, an 
      interesting model to study the molecular mechanisms ensuing the targeting of 
      5-HT(1) autoreceptors to somas and dendrites.
FAU - Rumajogee, Prakasham
AU  - Rumajogee P
AD  - Laboratoire de Neurobiologie des Signaux Intercellulaires, CNRS UMR 7101, 
      Universite Pierre et Marie Curie, 75005 Paris, France.
FAU - Verge, Daniel
AU  - Verge D
FAU - Hamon, Michel
AU  - Hamon M
FAU - Miquel, Marie-Christine
AU  - Miquel MC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060331
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MAP2 protein, rat)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Receptor, Serotonin, 5-HT1B)
RN  - 112692-38-3 (Receptor, Serotonin, 5-HT1A)
RN  - 333DO1RDJY (Serotonin)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cell Line
MH  - Cyclic AMP/*pharmacology
MH  - Dendrites/metabolism
MH  - Immunohistochemistry/methods
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neurons/*cytology/drug effects
MH  - Raphe Nuclei/*cytology
MH  - Rats
MH  - Receptor, Serotonin, 5-HT1A/*metabolism
MH  - Receptor, Serotonin, 5-HT1B/*metabolism
MH  - Serotonin/*metabolism/pharmacology
EDAT- 2006/04/04 09:00
MHDA- 2006/08/11 09:00
CRDT- 2006/04/04 09:00
PHST- 2005/09/06 00:00 [received]
PHST- 2006/02/21 00:00 [revised]
PHST- 2006/02/22 00:00 [accepted]
PHST- 2006/04/04 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2006/04/04 09:00 [entrez]
AID - S0006-8993(06)00521-X [pii]
AID - 10.1016/j.brainres.2006.02.086 [doi]
PST - ppublish
SO  - Brain Res. 2006 Apr 26;1085(1):121-6. doi: 10.1016/j.brainres.2006.02.086. Epub 
      2006 Mar 31.