PMID- 16580397
OWN - NLM
STAT- MEDLINE
DCOM- 20060511
LR  - 20091119
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 85
IP  - 4
DP  - 2006 Apr
TI  - Aneuploidy screening in a coelomic sample from a missed abortion using sequential 
      fluorescence in situ hybridization.
PG  - 1059.e13-6
AB  - OBJECTIVE: To investigate the incidence of aneuploidy by using sequential 
      interphase fluorescence in situ hybridization (FISH) in an uncultured coelomic 
      fluid sample from a missed abortion. DESIGN: Case report. SETTING: Fetal medicine 
      and gynecology departments of two university hospitals. PATIENT(S): A 33-year-old 
      woman with a missed abortion. INTERVENTION(S): Molecular cytogenetic analysis was 
      performed on coelomic fluid cells and placental tissue by applying a multicolor 
      probe for chromosomes 13, 16, 18, 21, and 22 in a first layer of hybridization, 
      followed by a triple-color probe for chromosomes X, Y, and 18. MAIN OUTCOME 
      MEASURE(S): Analysis of limited number of coelomic cells by using sequential 
      FISH. RESULT(S): The results showed trisomy 13 in 92% of nuclei. Diploid cells 
      were observed at low levels (4%), but these were caused by maternal cell 
      contamination, as revealed after reprobing with the X,Y,18 triple-color probe 
      set. The result also was confirmed in the placental tissue. CONCLUSION(S): We 
      conclude that sequential FISH analysis can overcome problems associated with 
      limited cell numbers, accurately detect aneuploidy, and distinguish between 
      maternal cell contamination and genuine mosaicism in coelomic cells and placental 
      tissue from missed abortions. It also has the potential to be applied for rapid 
      aneuploidy screening in clinical cases after coelocentesis and to be used for 
      follow-up analysis of pregnancies established after preimplantation genetic 
      screening.
FAU - Chatzimeletiou, Katerina
AU  - Chatzimeletiou K
AD  - Harris Birthright Centre for Fetal Medicine, King's College Hospital, London, 
      United Kingdom. katerinachatzime@hotmail.com
FAU - Makrydimas, George
AU  - Makrydimas G
FAU - Nicolaides, Kypros H
AU  - Nicolaides KH
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Abortion, Missed/diagnosis/*genetics
MH  - Adult
MH  - *Aneuploidy
MH  - Female
MH  - Genetic Testing/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Placenta/physiology
MH  - Pregnancy
EDAT- 2006/04/04 09:00
MHDA- 2006/05/12 09:00
CRDT- 2006/04/04 09:00
PHST- 2005/05/24 00:00 [received]
PHST- 2005/09/09 00:00 [revised]
PHST- 2005/09/09 00:00 [accepted]
PHST- 2006/04/04 09:00 [pubmed]
PHST- 2006/05/12 09:00 [medline]
PHST- 2006/04/04 09:00 [entrez]
AID - S0015-0282(06)00011-2 [pii]
AID - 10.1016/j.fertnstert.2005.09.059 [doi]
PST - ppublish
SO  - Fertil Steril. 2006 Apr;85(4):1059.e13-6. doi: 10.1016/j.fertnstert.2005.09.059.