PMID- 16581304
OWN - NLM
STAT- MEDLINE
DCOM- 20070806
LR  - 20120327
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 143
IP  - 2
DP  - 2006 Jun
TI  - Hexokinase and glucose-6-phosphatase activity in woodchuck model of hepatitis 
      virus-induced hepatocellular carcinoma.
PG  - 225-31
AB  - 2-Deoxy-2-[(18)F]fluoro-D-glucose ([(18)F] FDG) is used for PET imaging of 
      woodchuck (Marmota monax) model of hepatocellular carcinoma (HCC). The usefulness 
      of FDG on this animal model needs to be validated according to the hypothesized 
      mechanisms. In this study, two key enzymes involved in glucose or [(18)F] FDG 
      metabolism, hexokinase (HK) and glucose-6-phophatase (G6Pase), were examined for 
      their enzymatic activities in the woodchuck models of HCC, which has not been 
      studied before. After dynamic PET scans, woodchuck liver tissue samples were 
      harvested and the homogenate was centrifuged. The supernatant was used for HK 
      activity assay and the microsomal pellet was used for G6Pase assay. HK and G6Pase 
      activities were measured by means of colorimetric reactions via kinetic and 
      end-point assays, respectively. Total protein content was measured by the 
      Bradford method and used to normalize all enzyme activities. HK and G6Pase 
      activities in woodchuck HCC will be used to correlate with in vivo PET imaging 
      data. The woodchuck model of HCC had significantly increased levels of HK in the 
      livers compared to the age-matching healthy woodchuck (7.96 +/- 1.27 vs. 2.74 +/- 
      0.66 mU/mg protein, P < 0.01) and significantly decreased levels of G6Pase 
      compared to healthy woodchuck (40.35 +/- 19.28 vs. 237.01 +/- 17.32 mU/mg 
      protein, P < 0.01), reflecting an increase in glycolysis. In addition, 
      significant differences were found in HK and G6Pase activities between HCC liver 
      region (HK: 7.96 +/- 1.27 mU/mg protein; G6Pase: 40.35 +/- 19.28 mU/mg protein) 
      and surrounding normal liver region (HK: 2.98 +/- 0.92 mU/mg protein; G6Pase: 
      140.87 +/- 30.62 mU/mg protein) in the same woodchuck model of HCC (P < 0.01). 
      Our study demonstrated an increased HK activity and a decreased G6Pase activity 
      in liver of the woodchuck models of HCC as compared to normal woodchuck liver.
FAU - Kuang, Yu
AU  - Kuang Y
AD  - Department of Biomedical Engineering, Case Western Reserve University, Cleveland, 
      OH 44106, USA.
FAU - Schomisch, Steve J
AU  - Schomisch SJ
FAU - Chandramouli, Visvanathan
AU  - Chandramouli V
FAU - Lee, Zhenghong
AU  - Lee Z
LA  - eng
GR  - CA095307/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060306
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 3.1.3.9 (Glucose-6-Phosphatase)
SB  - IM
MH  - Animals
MH  - Carcinoma, Hepatocellular/*enzymology/virology
MH  - Disease Models, Animal
MH  - Female
MH  - Glucose-6-Phosphatase/*metabolism
MH  - Hepatitis B Virus, Woodchuck/isolation & purification
MH  - Hexokinase/*metabolism
MH  - Liver/enzymology/virology
MH  - Liver Neoplasms/*enzymology/virology
MH  - Marmota/*metabolism/virology
EDAT- 2006/04/04 09:00
MHDA- 2007/08/07 09:00
CRDT- 2006/04/04 09:00
PHST- 2005/11/17 00:00 [received]
PHST- 2006/01/30 00:00 [revised]
PHST- 2006/02/12 00:00 [accepted]
PHST- 2006/04/04 09:00 [pubmed]
PHST- 2007/08/07 09:00 [medline]
PHST- 2006/04/04 09:00 [entrez]
AID - S1532-0456(06)00051-2 [pii]
AID - 10.1016/j.cbpc.2006.02.005 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2006 Jun;143(2):225-31. doi: 
      10.1016/j.cbpc.2006.02.005. Epub 2006 Mar 6.