PMID- 1659504
OWN - NLM
STAT- MEDLINE
DCOM- 19920102
LR  - 20190706
IS  - 0009-7330 (Print)
IS  - 0009-7330 (Linking)
VI  - 69
IP  - 6
DP  - 1991 Dec
TI  - Effects of purines and pyrimidines on the rat mesenteric arterial bed.
PG  - 1583-90
AB  - The effects of the purines, adenosine 5'-triphosphate (ATP) and guanosine 
      5'-triphosphate (GTP), and the pyrimidines, uridine 5'-triphosphate (UTP), 
      cytidine 5'-triphosphate (CTP), and thymidine 5'-triphosphate (TTP), on vascular 
      resistance were investigated in the rat mesenteric arterial bed. In preparations 
      at basal tone, these agents produced dose-related vasoconstriction with a potency 
      order of ATP greater than CTP greater than UTP much greater than TTP = GTP. When 
      tone was raised with norepinephrine (30 microM), these agents caused dose-related 
      vasodilatation with the potency order of UTP = ATP greater than TTP = GTP. CTP 
      did not elicit vasodilatation. Removal of the endothelium with sodium 
      deoxycholate resulted in an increased responsiveness of the mesenteric bed 
      preparation to the vasoconstrictor effects of each of the purines and pyrimidines 
      tested. The selective P2 X-purinoceptor-desensitizing agent alpha,beta-methylene 
      ATP inhibited vasoconstrictor responses to ATP and to CTP but had no effect on 
      vasoconstrictor responses elicited by UTP, TTP, and GTP. In raised-tone 
      preparations, vasodilator responses to ATP, UTP, TTP, and GTP were abolished 
      after removal of the endothelium with sodium deoxycholate. Responses to 
      acetylcholine were also abolished; those to sodium nitroprusside were unimpaired. 
      An inhibitor of the formation of nitric oxide from L-arginine, N 
      omega-nitro-L-arginine methyl ester (30 microM), which antagonizes responses 
      mediated by endothelium-derived relaxing factor (nitric oxide), attenuated 
      vasodilatation to ATP, UTP, and acetylcholine but not to sodium 
      nitroprusside.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Ralevic, V
AU  - Ralevic V
AD  - Department of Anatomy and Developmental Biology, University College London, UK.
FAU - Burnstock, G
AU  - Burnstock G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Circ Res
JT  - Circulation research
JID - 0047103
RN  - 0 (Purine Nucleotides)
RN  - 0 (Pyrimidine Nucleotides)
RN  - 0 (Receptors, Cell Surface)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 94ZLA3W45F (Arginine)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Arginine/analogs & derivatives/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - Mesenteric Arteries/*drug effects
MH  - NG-Nitroarginine Methyl Ester
MH  - Nitric Oxide/physiology
MH  - Purine Nucleotides/*pharmacology
MH  - Pyrimidine Nucleotides/*pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Receptors, Cell Surface/physiology
MH  - Splanchnic Circulation/drug effects
MH  - *Vascular Resistance/drug effects
MH  - Vasoconstriction/drug effects
EDAT- 1991/12/01 00:00
MHDA- 1991/12/01 00:01
CRDT- 1991/12/01 00:00
PHST- 1991/12/01 00:00 [pubmed]
PHST- 1991/12/01 00:01 [medline]
PHST- 1991/12/01 00:00 [entrez]
AID - 10.1161/01.res.69.6.1583 [doi]
PST - ppublish
SO  - Circ Res. 1991 Dec;69(6):1583-90. doi: 10.1161/01.res.69.6.1583.