PMID- 16595651
OWN - NLM
STAT- MEDLINE
DCOM- 20060807
LR  - 20220410
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 281
IP  - 22
DP  - 2006 Jun 2
TI  - Transcription factors, cAMP-responsive element modulator (CREM) and Tisp40, act 
      in concert in postmeiotic transcriptional regulation.
PG  - 15073-81
AB  - We previously isolated 80 TISP (transcript induced in spermiogenesis) genes whose 
      transcription is dramatically induced during spermiogenesis. Our analysis here of 
      the expression of these genes in the testis of the cAMP-responsive element 
      modulator (CREM)-null mouse revealed that 54 TISP genes are under the 
      transcriptional regulation of CREM. One CREM-regulated gene is TISP40, which 
      encodes a basic leucine zipper (bZip)-type transcription factor bearing a 
      transmembrane domain that generates the two proteins Tisp40alpha and Tisp40beta. 
      Both of these proteins function by binding to UPRE (unfolded protein-response 
      element) but do not recognize CRE motifs. We show here that Tisp40alpha mRNA is 
      generated under the direct transcriptional regulation of CREM. CREMtau and Tisp40 
      form a heterodimer, which functions through CRE but not through UPRE. 
      Furthermore, binding ability of CREM to CRE is dramatically up-regulated by 
      forming a heterodimer with Tisp40alphaDeltaTM, a truncated form of Tisp40alpha 
      that lacks the transmembrane domain. We confirmed that Tisp40 and CREM actually 
      bind to the Tisp40 promoter in vivo by chromatin immunoprecipitation assay. 
      Finally, we demonstrate that the Tisp40DeltaTM-CREMtau heterodimer acts as a 
      recruiter of HIRA, a histone chaperone, to CRE. Taken together, we propose that 
      Tisp40 is an important transcriptional regulator during spermiogenesis.
FAU - Nagamori, Ippei
AU  - Nagamori I
AD  - Department of Molecular Genetics, Research Institute for Microbial Diseases, 
      Osaka University, Yamadaoka 3-1, Suita City, Osaka 565-0871, Japan.
FAU - Yomogida, Kentaro
AU  - Yomogida K
FAU - Adams, Peter D
AU  - Adams PD
FAU - Sassone-Corsi, Paolo
AU  - Sassone-Corsi P
FAU - Nojima, Hiroshi
AU  - Nojima H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060404
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Creb3 protein, mouse)
RN  - 0 (Crem protein, mouse)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Hira protein, mouse)
RN  - 0 (Histone Chaperones)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 135844-64-3 (Cyclic AMP Response Element Modulator)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Cell Cycle Proteins/metabolism
MH  - Cyclic AMP Response Element Modulator/chemistry/deficiency/genetics/*metabolism
MH  - Cyclic AMP Response Element-Binding Protein/chemistry/genetics/*metabolism
MH  - DNA/genetics/metabolism
MH  - Dimerization
MH  - HeLa Cells
MH  - Histone Chaperones
MH  - Humans
MH  - In Vitro Techniques
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Spermatogenesis/*genetics
MH  - Transcription Factors/metabolism
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 2006/04/06 09:00
MHDA- 2006/08/08 09:00
CRDT- 2006/04/06 09:00
PHST- 2006/04/06 09:00 [pubmed]
PHST- 2006/08/08 09:00 [medline]
PHST- 2006/04/06 09:00 [entrez]
AID - S0021-9258(20)55951-6 [pii]
AID - 10.1074/jbc.M602051200 [doi]
PST - ppublish
SO  - J Biol Chem. 2006 Jun 2;281(22):15073-81. doi: 10.1074/jbc.M602051200. Epub 2006 
      Apr 4.