PMID- 16595720
OWN - NLM
STAT- MEDLINE
DCOM- 20061003
LR  - 20131121
IS  - 1470-1626 (Print)
IS  - 1470-1626 (Linking)
VI  - 131
IP  - 4
DP  - 2006 Apr
TI  - Presence and regulation of messenger ribonucleic acids encoding components of the 
      class II major histocompatibility complex-associated antigen processing pathway 
      in the bovine corpus luteum.
PG  - 689-98
AB  - Luteal cells express class II major histocompatibility complex (MHC) molecules 
      and can stimulate T lymphocyte proliferation in vitro. However, it is unknown 
      whether luteal cells express the intracellular components necessary to process 
      the peptides presented by class II MHC molecules. The objective of the present 
      study was to examine the expression and regulation of three major class 
      II-associated antigen processing components--class II MHC-associated invariant 
      chain (Ii), DMalpha and DMbeta--in luteal tissue. Corpora lutea were collected 
      early in the estrous cycle, during midcycle and late in the estrous cycle, and at 
      various times following administration of a luteolytic dose of prostaglandin 
      F(2alpha) (PGF(2alpha)) to the cow. Northern analysis revealed the presence of 
      mRNA encoding each of the class II MHC-associated antigen processing proteins in 
      luteal tissue. Ii mRNA concentrations did not change during the estrous cycle, 
      whereas DMalpha and DMbeta mRNA concentrations were highest in midcycle luteal 
      tissue compared with either early or late luteal tissue. Tumor necrosis 
      factor-alpha (TNF-alpha) reduced DMalpha mRNA concentrations in cultured luteal 
      cells in the presence of LH or PGF(2alpha). DMalpha and DMbeta mRNA were also 
      present in highly enriched cultures of luteal endothelial (CLENDO) cells, and 
      DMalpha mRNA concentrations were greater in CLENDO cultures compared with mixed 
      luteal cell cultures. Expression of invariant chain, DMalpha and DMbeta genes 
      indicates that cells within the corpus luteum express the minimal requirements to 
      act as functional antigen-presenting cells, and the observation that CLENDO cells 
      are a source of DMalpha and DMbeta mRNA indicates that non-immune cells within 
      the corpus luteum may function as antigen-presenting cells.
FAU - Cannon, Matthew J
AU  - Cannon MJ
AD  - Department of Animal Sciences, The Ohio State University/Ohio Agricultural 
      Research and Development Center, 1680 Madison Avenue, Wooster, Ohio 44691, USA.
FAU - Davis, John S
AU  - Davis JS
FAU - Pate, Joy L
AU  - Pate JL
LA  - eng
GR  - HD37550/HD/NICHD NIH HHS/United States
GR  - HD48754/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (invariant chain)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - B7IN85G1HY (Dinoprost)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/*immunology
MH  - Antigens, Differentiation, B-Lymphocyte/*genetics
MH  - Blotting, Northern/methods
MH  - Cattle
MH  - Cells, Cultured
MH  - Corpus Luteum/*immunology
MH  - Dinoprost/pharmacology
MH  - Endothelial Cells/immunology
MH  - Estrous Cycle
MH  - Female
MH  - *Gene Expression Regulation, Developmental/drug effects
MH  - Histocompatibility Antigens Class II/*genetics
MH  - Luteinizing Hormone/pharmacology
MH  - RNA, Messenger/*analysis/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/*genetics
MH  - Stimulation, Chemical
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2006/04/06 09:00
MHDA- 2006/10/04 09:00
CRDT- 2006/04/06 09:00
PHST- 2006/04/06 09:00 [pubmed]
PHST- 2006/10/04 09:00 [medline]
PHST- 2006/04/06 09:00 [entrez]
AID - 131/4/689 [pii]
AID - 10.1530/rep.1.00906 [doi]
PST - ppublish
SO  - Reproduction. 2006 Apr;131(4):689-98. doi: 10.1530/rep.1.00906.