PMID- 16596121
OWN - NLM
STAT- MEDLINE
DCOM- 20070917
LR  - 20200730
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 27
IP  - 1
DP  - 2007 Jan
TI  - Therapeutic benefit of bone marrow stromal cells administered 1 month after 
      stroke.
PG  - 6-13
AB  - Bone marrow stromal cells (BMSCs) facilitate functional recovery in rats after 
      stroke when administered acutely (1 day) or subacutely (7 days). In this study, 
      we postponed the time of cell transplantation to 1 month after stroke. Female 
      retired breeder rats were subjected to 2 h of middle cerebral artery occlusion 
      (MCAo). Male BMSCs (3 x 10(6)) or phosphate-buffered saline were administered 
      intravenously, and the animals were killed 3 months later. An additional 
      population of nontreated rats was killed at 1 month after MCAo. Significant 
      recovery of behavior was found in BMSC-treated rats beginning at 1 month after 
      cell injection in the modified neurologic severity score test and the 
      adhesive-removal test compared with control animals (P<0.05). In situ 
      hybridization showed that BMSCs survived and preferentially localized to the 
      ipsilateral hemisphere. Double staining revealed that approximately 13% and 6% 
      Y-chromosome-positive cells expressed the astrocyte marker, glial fibrillary 
      acidic protein, and the neuronal marker, microtubule-associated protein-2, 
      respectively. In addition, BMSC treatment reduced scar thickness, and increased 
      the number of proliferating cells and oligodendrocyte precursor cells along the 
      subventricular zone in the ipsilateral hemisphere. Expression of the chemokine 
      stromal-cell-derived factor-1 (SDF-1) was significantly increased along the 
      ischemic boundary zone compared with the corresponding areas in the contralateral 
      hemisphere at 1 month and 4 months (P<0.01) after stroke. The SDF-1 receptor, 
      CXC-chemokine receptor-4 (CXCR4), was expressed in BMSCs both in vitro and in 
      vivo. Our data show that the time window of BMSC therapy is at least 1 month 
      after stroke; the interaction of SDF-1/CXCR4 may contribute to the trafficking of 
      transplanted BMSCs.
FAU - Shen, Li Hong
AU  - Shen LH
AD  - Department of Neurology, Henry Ford Health Sciences Center, Henry Ford Hospital, 
      Detroit, Michigan 48202, USA.
FAU - Li, Yi
AU  - Li Y
FAU - Chen, Jieli
AU  - Chen J
FAU - Zacharek, Alex
AU  - Zacharek A
FAU - Gao, Qi
AU  - Gao Q
FAU - Kapke, Allissa
AU  - Kapke A
FAU - Lu, Mei
AU  - Lu M
FAU - Raginski, Kim
AU  - Raginski K
FAU - Vanguri, Padmayathy
AU  - Vanguri P
FAU - Smith, Alan
AU  - Smith A
FAU - Chopp, Michael
AU  - Chopp M
LA  - eng
GR  - P01 NS042345/NS/NINDS NIH HHS/United States
GR  - R01 NS045041/NS/NINDS NIH HHS/United States
GR  - P01 NS23393/NS/NINDS NIH HHS/United States
GR  - R01 NS45041/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060405
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Nptn protein, rat)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Biomarkers
MH  - *Bone Marrow Transplantation
MH  - Chemokine CXCL12
MH  - Chemokines, CXC/blood
MH  - Female
MH  - Immunoglobulins/blood
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Infarction, Middle Cerebral Artery/pathology
MH  - Male
MH  - Membrane Glycoproteins/blood
MH  - Physical Stimulation
MH  - Psychomotor Performance/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, CXCR4/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stroke/pathology/psychology/*therapy
MH  - Stromal Cells/*transplantation
EDAT- 2006/04/06 09:00
MHDA- 2007/09/18 09:00
CRDT- 2006/04/06 09:00
PHST- 2006/04/06 09:00 [pubmed]
PHST- 2007/09/18 09:00 [medline]
PHST- 2006/04/06 09:00 [entrez]
AID - 9600311 [pii]
AID - 10.1038/sj.jcbfm.9600311 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 2007 Jan;27(1):6-13. doi: 10.1038/sj.jcbfm.9600311. 
      Epub 2006 Apr 5.