PMID- 16596626
OWN - NLM
STAT- MEDLINE
DCOM- 20060925
LR  - 20061115
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 94
IP  - 6
DP  - 2006 Aug 20
TI  - Population dynamics and in situ kinetics of nitrifying bacteria in autotrophic 
      nitrifying biofilms as determined by real-time quantitative PCR.
PG  - 1111-21
AB  - Population dynamics of ammonia-oxidizing bacteria (AOB) and uncultured 
      Nitrospira-like nitrite-oxidizing bacteria (NOB) dominated in autotrophic 
      nitrifying biofilms were determined by using real-time quantitative polymerase 
      chain reaction (RTQ-PCR) and fluorescence in situ hybridization (FISH). Although 
      two quantitative techniques gave the comparable results, the RTQ-PCR assay was 
      easier and faster than the FISH technique for quantification of both nitrifying 
      bacteria in dense microcolony-forming nitrifying biofilms. Using this RTQ-PCR 
      assay, we could successfully determine the maximum specific growth rate (mu = 
      0.021/h) of uncultured Nitrospira-like NOB in the suspended enrichment culture. 
      The population dynamics of nitrifying bacteria in the biofilm revealed that once 
      they formed the biofilm, the both nitrifying bacteria grew slower than in 
      planktonic cultures. We also calculated the spatial distributions of average 
      specific growth rates of both nitrifying bacteria in the biofilm based on the 
      concentration profiles of NH4+, NO2-, and O2, which were determined by 
      microelectrodes, and the double-Monod model. This simple model estimation could 
      explain the stratified spatial distribution of AOB and Nitrospira-like NOB in the 
      biofilm. The combination of culture-independent molecular techniques and 
      microelectrode measurements is a very powerful approach to analyze the in situ 
      kinetics and ecophysiology of nitrifying bacteria including uncultured 
      Nitrospira-like NOB in complex biofilm communities.
CI  - Copyright 2006 Wiley Periodicals, Inc.
FAU - Kindaichi, Tomonori
AU  - Kindaichi T
AD  - Department of Social and Environmental Engineering, Graduate School of 
      Engineering, Hiroshima University, Higashihiroshima, Japan.
FAU - Kawano, Yoshiko
AU  - Kawano Y
FAU - Ito, Tsukasa
AU  - Ito T
FAU - Satoh, Hisashi
AU  - Satoh H
FAU - Okabe, Satoshi
AU  - Okabe S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
SB  - IM
MH  - Bacteria/cytology/*isolation & purification/*metabolism
MH  - Biofilms/classification/*growth & development
MH  - Bioreactors/*microbiology
MH  - Cell Culture Techniques/methods
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Colony Count, Microbial/*methods
MH  - Computer Simulation
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Kinetics
MH  - *Models, Biological
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
EDAT- 2006/04/06 09:00
MHDA- 2006/09/26 09:00
CRDT- 2006/04/06 09:00
PHST- 2006/04/06 09:00 [pubmed]
PHST- 2006/09/26 09:00 [medline]
PHST- 2006/04/06 09:00 [entrez]
AID - 10.1002/bit.20926 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2006 Aug 20;94(6):1111-21. doi: 10.1002/bit.20926.