PMID- 16597899
OWN - NLM
STAT- MEDLINE
DCOM- 20061114
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 44
IP  - 4
DP  - 2006 Apr
TI  - A 24-hour screening protocol for identification of vancomycin-resistant 
      Enterococcus faecium.
PG  - 1578-80
AB  - We describe a 24-h protocol for the identification of patients who are positive 
      for vancomycin-resistant Enterococcus faecium (VRE), using stool and rectal swab 
      samples and VRE screening broth, automated DNA extraction, and real-time PCR for 
      vanA and vanB genes. Compared to conventional screening methods, this protocol 
      had a high sensitivity and specificity and a negative predictive value.
FAU - Drews, Steven J
AU  - Drews SJ
AD  - Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, 555 
      University Ave., Room 3654, Toronto, Ontario M5G 1X8, Canada.
FAU - Johnson, Grant
AU  - Johnson G
FAU - Gharabaghi, Farhad
AU  - Gharabaghi F
FAU - Roscoe, Margaret
AU  - Roscoe M
FAU - Matlow, Anne
AU  - Matlow A
FAU - Tellier, Raymond
AU  - Tellier R
FAU - Richardson, Susan E
AU  - Richardson SE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Bacterial Proteins)
RN  - 0 (VanA ligase, Bacteria)
RN  - 0 (VanB protein, Enterococcus)
RN  - 6Q205EH1VU (Vancomycin)
RN  - EC 6.1.- (Carbon-Oxygen Ligases)
SB  - IM
MH  - Automation
MH  - Bacterial Proteins/*genetics
MH  - Carbon-Oxygen Ligases/*genetics
MH  - Enterococcus faecium/drug effects/genetics/*isolation & purification
MH  - Feces/microbiology
MH  - Gram-Positive Bacterial Infections/diagnosis/*microbiology
MH  - Humans
MH  - Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
MH  - Vancomycin/pharmacology
MH  - Vancomycin Resistance/*genetics
PMC - PMC1448643
EDAT- 2006/04/07 09:00
MHDA- 2006/11/15 09:00
PMCR- 2006/08/01
CRDT- 2006/04/07 09:00
PHST- 2006/04/07 09:00 [pubmed]
PHST- 2006/11/15 09:00 [medline]
PHST- 2006/04/07 09:00 [entrez]
PHST- 2006/08/01 00:00 [pmc-release]
AID - 44/4/1578 [pii]
AID - 2141-05 [pii]
AID - 10.1128/JCM.44.4.1578-1580.2006 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2006 Apr;44(4):1578-80. doi: 10.1128/JCM.44.4.1578-1580.2006.