PMID- 16598010 OWN - NLM STAT- MEDLINE DCOM- 20060628 LR - 20181224 IS - 0099-2240 (Print) IS - 1098-5336 (Electronic) IS - 0099-2240 (Linking) VI - 72 IP - 4 DP - 2006 Apr TI - Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities. PG - 3016-20 AB - Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. This study overcomes these limitations by combining FISH and spectral imaging. FAU - Ainsworth, T D AU - Ainsworth TD AD - Centre for Marine Studies, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia. t.ainsworth@uq.edu.au FAU - Fine, M AU - Fine M FAU - Blackall, L L AU - Blackall LL FAU - Hoegh-Guldberg, O AU - Hoegh-Guldberg O LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Appl Environ Microbiol JT - Applied and environmental microbiology JID - 7605801 RN - 0 (Carbocyanines) RN - 0 (Fluorescent Dyes) RN - 0 (cyanine dye 3) SB - IM MH - Animals MH - Anthozoa/*microbiology/parasitology/*physiology MH - Bacteria/growth & development/*metabolism MH - Carbocyanines/*analysis MH - Fluorescence MH - Fluorescent Dyes/*analysis MH - Image Processing, Computer-Assisted MH - In Situ Hybridization, Fluorescence/*methods MH - Microscopy, Confocal MH - Snails/physiology MH - Symbiosis PMC - PMC1449077 EDAT- 2006/04/07 09:00 MHDA- 2006/06/29 09:00 PMCR- 2006/08/01 CRDT- 2006/04/07 09:00 PHST- 2006/04/07 09:00 [pubmed] PHST- 2006/06/29 09:00 [medline] PHST- 2006/04/07 09:00 [entrez] PHST- 2006/08/01 00:00 [pmc-release] AID - 72/4/3016 [pii] AID - 2862-05 [pii] AID - 10.1128/AEM.72.4.3016-3020.2006 [doi] PST - ppublish SO - Appl Environ Microbiol. 2006 Apr;72(4):3016-20. doi: 10.1128/AEM.72.4.3016-3020.2006.