PMID- 16600979
OWN - NLM
STAT- MEDLINE
DCOM- 20061114
LR  - 20220317
IS  - 0923-7534 (Print)
IS  - 0923-7534 (Linking)
VI  - 17
IP  - 6
DP  - 2006 Jun
TI  - Vaccination of colorectal cancer patients with CEA-loaded dendritic cells: 
      antigen-specific T cell responses in DTH skin tests.
PG  - 974-80
AB  - BACKGROUND: Dendritic cells (DCs) are the professional antigen-presenting cells 
      of the immune system. As such they are currently used in clinical vaccination 
      protocols in cancer patients. PATIENTS AND METHODS: We evaluated the ability of 
      mature DCs pulsed with carcinoembryonic antigen (CEA)-peptide to induce 
      CEA-specific T cell responses in patients with resectable liver metastases from 
      colorectal cancer. CEA-specific T cell reactivity was monitored in peripheral 
      blood, biopsies of vaccination sites and post-treatment DTH skin tests, and when 
      available also in resected abdominal lymph nodes and tumor tissue. RESULTS: Ten 
      patients were vaccinated intradermally and intravenously with CEA-peptide pulsed 
      mature DCs three times prior to resection of liver metastases. High numbers of 
      CEA-specific T cells were detected in post-treatment DTH biopsies in seven out of 
      10 patients, which produced high amounts of interferon (IFN)-gamma upon 
      stimulation with CEA-loaded target cells. These responses were not found in 
      biopsies of first vaccination sites, indicating a de novo T cell induction or at 
      least a strong potentiation by the vaccine. In addition, CEA-specific T cells 
      were detected in a resected lymph node in one patient, but not in peripheral 
      blood or tumor tissue. CONCLUSIONS: Vaccination with CEA-peptide loaded mature 
      DCs induced potent CEA-specific T cell responses in advanced colorectal cancer 
      patients. In this study, antigen-specific T cell responses were readily detected 
      in DTH skin tests, much less in abdominal lymph nodes, and not in peripheral 
      blood and tumor tissue.
FAU - Lesterhuis, W J
AU  - Lesterhuis WJ
AD  - Department of Medical Oncology, Nijmegen Centre for Molecular Life Sciences and 
      Radboud University Nijmegen Medical Centre, The Netherlands.
FAU - de Vries, I J M
AU  - de Vries IJ
FAU - Schuurhuis, D H
AU  - Schuurhuis DH
FAU - Boullart, A C I
AU  - Boullart AC
FAU - Jacobs, J F M
AU  - Jacobs JF
FAU - de Boer, A J
AU  - de Boer AJ
FAU - Scharenborg, N M
AU  - Scharenborg NM
FAU - Brouwer, H M H
AU  - Brouwer HM
FAU - van de Rakt, M W M M
AU  - van de Rakt MW
FAU - Figdor, C G
AU  - Figdor CG
FAU - Ruers, T J
AU  - Ruers TJ
FAU - Adema, G J
AU  - Adema GJ
FAU - Punt, C J A
AU  - Punt CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060406
PL  - England
TA  - Ann Oncol
JT  - Annals of oncology : official journal of the European Society for Medical 
      Oncology
JID - 9007735
RN  - 0 (Cancer Vaccines)
RN  - 0 (Carcinoembryonic Antigen)
SB  - IM
MH  - Adult
MH  - *Cancer Vaccines/administration & dosage
MH  - Carcinoembryonic Antigen/*immunology
MH  - Colorectal Neoplasms/*immunology/pathology
MH  - Dendritic Cells/*transplantation
MH  - Drug Administration Schedule
MH  - Humans
MH  - Hypersensitivity, Delayed/*immunology
MH  - Liver Neoplasms/*immunology/*secondary/surgery
MH  - Lymph Nodes/immunology/pathology
MH  - Lymphatic Metastasis
MH  - Lymphocyte Activation
MH  - Monitoring, Immunologic
MH  - Patient Selection
MH  - Skin Tests
MH  - T-Lymphocytes/*immunology
MH  - Transplantation, Autologous
MH  - Treatment Outcome
EDAT- 2006/04/08 09:00
MHDA- 2006/11/15 09:00
CRDT- 2006/04/08 09:00
PHST- 2006/04/08 09:00 [pubmed]
PHST- 2006/11/15 09:00 [medline]
PHST- 2006/04/08 09:00 [entrez]
AID - S0923-7534(19)43578-3 [pii]
AID - 10.1093/annonc/mdl072 [doi]
PST - ppublish
SO  - Ann Oncol. 2006 Jun;17(6):974-80. doi: 10.1093/annonc/mdl072. Epub 2006 Apr 6.