PMID- 1660188
OWN - NLM
STAT- MEDLINE
DCOM- 19920109
LR  - 20220225
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 254
IP  - 5037
DP  - 1991 Dec 6
TI  - Regulation of phagocyte oxygen radical production by the GTP-binding protein Rac 
      2.
PG  - 1512-5
AB  - A major action of the microbicidal system of human neutrophils is the formation 
      of superoxide anion (O2-) by a multicomponent oxidase that transfers electrons 
      from the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) to 
      molecular oxygen. The mechanism of assembly and activation of the oxidase from 
      its cytosolic and membrane-bound components is unknown, but may require the 
      activity of a guanosine 5'-triphosphate (GTP)-binding component. A cytosolic 
      GTP-binding protein (Gox) that regulates the NADPH oxidase of neutrophils was 
      identified. Gox was purified and shown to augment the rate of O2- production in a 
      cell-free oxidase activation system. Sequence analysis of peptide fragments from 
      Gox identified it as Rac 2, a member of the Ras superfamily of GTP-binding 
      proteins. Antibody to a peptide derived from the COOH-terminus of Rac 2 inhibited 
      O2- generation in a concentration-dependent manner. These results suggest that 
      Rac 2 is a regulatory component of the human neutrophil NADPH oxidase, and 
      provide new insights into the mechanism by which this oxygen radical-generating 
      system is regulated.
FAU - Knaus, U G
AU  - Knaus UG
AD  - Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.
FAU - Heyworth, P G
AU  - Heyworth PG
FAU - Evans, T
AU  - Evans T
FAU - Curnutte, J T
AU  - Curnutte JT
FAU - Bokoch, G M
AU  - Bokoch GM
LA  - eng
GR  - AI24838/AI/NIAID NIH HHS/United States
GR  - GM39434/GM/NIGMS NIH HHS/United States
GR  - HL48008/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Free Radicals)
RN  - 11062-77-4 (Superoxides)
RN  - EC 1.6.- (NADH, NADPH Oxidoreductases)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
SB  - IM
GS  - Rac1
GS  - Rac2
MH  - Amino Acid Sequence
MH  - Free Radicals
MH  - GTP-Binding Proteins/*physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Molecular Sequence Data
MH  - NADH, NADPH Oxidoreductases/*metabolism
MH  - NADPH Oxidases
MH  - Neutrophils/*physiology
MH  - *Respiratory Burst
MH  - Superoxides/metabolism
MH  - rac GTP-Binding Proteins
EDAT- 1991/12/06 00:00
MHDA- 1991/12/06 00:01
CRDT- 1991/12/06 00:00
PHST- 1991/12/06 00:00 [pubmed]
PHST- 1991/12/06 00:01 [medline]
PHST- 1991/12/06 00:00 [entrez]
AID - 10.1126/science.1660188 [doi]
PST - ppublish
SO  - Science. 1991 Dec 6;254(5037):1512-5. doi: 10.1126/science.1660188.