PMID- 16603127
OWN - NLM
STAT- MEDLINE
DCOM- 20060623
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 344
IP  - 1
DP  - 2006 May 26
TI  - Expression profilings of 39 genes selected by ANOVA could separate precursors of 
      murine dendritic cells and macrophages.
PG  - 438-45
AB  - Dendritic cells (DCs) and macrophages share some stages in the development and 
      function of antigen presentation. But it is difficult to separate them from their 
      precursors. We used one-way ANOVA (analysis of variances) on murine expression 
      profilings of several hematopoietic cells associated with DCs and macrophages to 
      find the genes with great differences across the cell groups. These groups were 
      the DCs from spleen, cultivated DCs, DC precursors, DC progenitors, DC progenitor 
      cell lines, hematopoietic stem cell (HSC), and bone marrow-derived macrophages. 
      The data of expression profilings were all downloaded from GEO and ArrayExpress 
      database. After the normalization of 11 housekeeping genes across 42 arrays, we 
      got 39 genes (44 probesets) by analysis of one-way ANOVA (Bonferroni step-down) 
      with p values cutoff of 0.05. These genes (probesets) could separate the 
      hematopoietic cells well by the methods of unsupervised hierarchical clustering 
      and principal component analysis (PCA). The class prediction also indicated that 
      these genes could separate the precursors of DC and macrophages with 20 arrays 
      composed of 5 cell types with the same normalization. The accuracy rate of class 
      prediction was 90% (18/20). The genes selected by one-way ANOVA included those of 
      MHC (major histocompatibility complex) and defense of immunity, cell adhesion, 
      chemokine or its receptors, and transcription factors. The results indicated that 
      these 39 genes could separate precursors of DC and macrophages very clearly. It 
      was suggested that these genes might represent some important molecules that 
      related with the precursors of DCs and macrophages, and were worthy for further 
      study.
FAU - Zhou, Shixin
AU  - Zhou S
AD  - Chien-Shiung Wu Laboratory, Department of Biological Science and Medical 
      Engineering, Southeast University, Nanjing 210096, China.
FAU - Liu, Yuqin
AU  - Liu Y
FAU - Bo, Hong
AU  - Bo H
FAU - Bian, Xiaocui
AU  - Bian X
FAU - Xia, Xiaojun
AU  - Xia X
FAU - Lin, Changsheng
AU  - Lin C
FAU - Wong, Vincent Wai-Sun
AU  - Wong VW
FAU - Lu, Zuhong
AU  - Lu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060417
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*genetics
MH  - Cells, Cultured
MH  - Cluster Analysis
MH  - Dendritic Cells/*cytology/metabolism
MH  - Gene Expression
MH  - *Gene Expression Profiling
MH  - Immunity/genetics
MH  - Macrophages/*cytology/metabolism
MH  - Mice
MH  - Stem Cells/*cytology/metabolism
EDAT- 2006/04/11 09:00
MHDA- 2006/06/24 09:00
CRDT- 2006/04/11 09:00
PHST- 2006/02/26 00:00 [received]
PHST- 2006/03/06 00:00 [accepted]
PHST- 2006/04/11 09:00 [pubmed]
PHST- 2006/06/24 09:00 [medline]
PHST- 2006/04/11 09:00 [entrez]
AID - S0006-291X(06)00543-2 [pii]
AID - 10.1016/j.bbrc.2006.03.125 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 May 26;344(1):438-45. doi: 
      10.1016/j.bbrc.2006.03.125. Epub 2006 Apr 17.