PMID- 16604110
OWN - NLM
STAT- MEDLINE
DCOM- 20060831
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 49
IP  - 3
DP  - 2006 Mar
TI  - Chromosome elimination in germ line-soma differentiation of Acricotopus lucidus 
      (Diptera, Chironomidae).
PG  - 269-74
AB  - During germ line-soma differentiation in early syncytial embryonic development of 
      the chironomid Acricotopus lucidus, a complement of supernumerary chromosomes, 
      the so-called germ line limited chromosomes (Ks), is excluded from the future 
      somatic nuclei in the course of elimination mitoses. The Ks lag behind in the 
      equatorial plane, while the somatic chromosomes (Ss) segregate equally. After 
      elimination mitoses, the Ks are only present in the pole cells, the primary germ 
      cells. In the divisions before their elimination, the Ks frequently showed 
      delayed separation of sister chromatids with high-frequency formation of 
      anaphasic bridges and lagging in pole movement as detected in 
      4',6-diamidino-2-phenylindole (DAPI)-stained squash preparations of early 
      embryos. To determine if all of the Ks are eliminated in one step during a single 
      mitosis, a fluorescence in situ hybridization (FISH) analysis of early embryonic 
      divisions was performed using probes of germ line specific repetitive DNA 
      sequences, which specifically label the Ks in their centromeric regions. In most 
      cases, all of the Ks are lost in one mitosis; however, occasionally one or 
      several of the Ks can escape their elimination by segregating and moving poleward 
      together with the Ss. The escaping Ks will then be eliminated in one of the 
      following mitoses. This clearly indicates that the specific conditions to 
      eliminate Ks are not restricted to only one division. Possible mechanisms of 
      elimination of Ks are discussed.
FAU - Staiber, Wolfgang
AU  - Staiber W
AD  - Institute of Genetics, University of Hohenheim, Garbenstrasse 30, D-70599 
      Stuttgart, Germany. wstaiber@uni-hohenheim.de
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cell Nucleus Division/physiology
MH  - Chironomidae/cytology/*embryology/*genetics
MH  - *Chromosomes
MH  - Embryo, Nonmammalian
MH  - Fluorescent Dyes
MH  - *Germ Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles
MH  - Mitosis
EDAT- 2006/04/11 09:00
MHDA- 2006/09/01 09:00
CRDT- 2006/04/11 09:00
PHST- 2006/04/11 09:00 [pubmed]
PHST- 2006/09/01 09:00 [medline]
PHST- 2006/04/11 09:00 [entrez]
AID - g05-103 [pii]
AID - 10.1139/g05-103 [doi]
PST - ppublish
SO  - Genome. 2006 Mar;49(3):269-74. doi: 10.1139/g05-103.