PMID- 16608072
OWN - NLM
STAT- MEDLINE
DCOM- 20070327
LR  - 20131121
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 37
IP  - 2
DP  - 2006 Mar
TI  - [A preliminary study on the mechanism of neurotoxicity of MDMA--oxidative stress 
      harm].
PG  - 191-5
AB  - OBJECTIVE: Establishing a long-term neurotoxic model to explore the mechanism of 
      neurotoxicity of 3,4-methylenedioxymethamphetamine (MDMA) and the putative 
      protection conferred by Vit C against oxidative stress harm. METHODS: Male Wistar 
      rats were randomly assigned to control group (A) and MDMA treatment groups(B, C, 
      D, E). Rats of group B were given MDMA 20 mg/kg; groups C, D, E were given Vit C 
      250 mg/kg 30 min before administration of MDMA (Vit C 30 min group) and 3 h (Vit 
      C 3 h group) and 5 h (VitC 5 h group) after administration of MDMA, respectively. 
      Rats of control group were treated with the same volume of saline. Concentrations 
      of ATP and ADP in brain cortex and 5-HT in hippocampus and occipital cortex were 
      measured by high perfor-mance liquid chromatography; the expression of SERT mRNA 
      was detected by in situ hybridization; and the expression of protein GFAP was 
      detected by immunohisto-chemistry. RESULTS: hours after MDMA treatment, the 
      concentration of ATP in brain cortex was lessened, compared with control (P 
      <0.05). On the 7th day after MDMA treatment, the concentration of 5-HT in rat 
      hippocampus and occipital cortex was decreased, compared with control (P<0.05). 
      The expression of SERT mRNA in hippocampus was decreased, whereas the expression 
      of GFAP in brain tissue was increased (P<0.05). The adminstration of Vit C 30 min 
      before MDMA treatment and 3 h after MDMA treatment did not curb the decrease of 
      ATP, 5-HT and the expression of SERT mRNA, but Vit C administrated 5 h after MDMA 
      treatment could curb the decrease of ATP and the functional markers of 5-HT. And 
      Vit C given at three time points did downregulate the GFAP expression. 
      CONCLUSION: MDMA could deplete the direct energetic substance ATP. MDMA could 
      exert neurotoxic effect on 5-HT system. Vit C given 5 h after MDMA administration 
      could provide neuroprotection for ATP and 5-HT system.
FAU - Li, Su-Xia
AU  - Li SX
AD  - Center of Psychology Health, West China Hospital, Sichuan University, Chengdu 
      610041, China.
FAU - Sun, Ai-Min
AU  - Sun AM
FAU - Wang, Xue
AU  - Wang X
FAU - Li, Jing
AU  - Li J
FAU - Peng, Zu-Gui
AU  - Peng ZG
FAU - Kuang, Wei-Hong
AU  - Kuang WH
FAU - Huang, Ming-Sheng
AU  - Huang MS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Hallucinogens)
RN  - 0 (RNA, Messenger)
RN  - 333DO1RDJY (Serotonin)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Adenosine Diphosphate/metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Ascorbic Acid/pharmacology
MH  - Brain/*metabolism
MH  - Hallucinogens/*toxicity
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*toxicity
MH  - *Oxidative Stress
MH  - RNA, Messenger/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Serotonin/*metabolism
EDAT- 2006/04/13 09:00
MHDA- 2007/03/28 09:00
CRDT- 2006/04/13 09:00
PHST- 2006/04/13 09:00 [pubmed]
PHST- 2007/03/28 09:00 [medline]
PHST- 2006/04/13 09:00 [entrez]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Mar;37(2):191-5.