PMID- 16608393
OWN - NLM
STAT- MEDLINE
DCOM- 20060531
LR  - 20220408
IS  - 1549-1684 (Print)
IS  - 1549-1684 (Linking)
VI  - 9
IP  - 1
DP  - 2006 Spring
TI  - Glucose-induced replicative senescence in mesenchymal stem cells.
PG  - 31-5
AB  - Mesenchymal stem cells (MSCs) show great promise for use in a variety of 
      cell-based therapies. Because isolated primary mesenchymal stem cells are low in 
      numbers, in vitro expansion is necessary. However, the expansion potential is 
      limited and in vitro aging leads to loss of multipotency and replicative 
      senescence. Stress induced by culture conditions is likely to be a major cause of 
      replicative senescence and reduced multipotency of MSC and optimization of 
      culture conditions might be able to reduce this. Caloric restriction (CR) is the 
      only established method to delay aging and extend lifespan. In vitro caloric 
      restriction experiments are rare, but have demonstrated beneficial effects. 
      Therefore, we investigated the effect of culture medium glucose concentration on 
      the proliferative and differentiation potential of mesenchymal stem cells. 
      Reduction in glucose concentrations led to decreased apoptosis and an increased 
      rate of MSC proliferation and increased the number and size of fibroblastic 
      colonies in the colony-forming unit assay.
FAU - Stolzing, Alexandra
AU  - Stolzing A
AD  - Kroto Research Institute, University of Sheffield, Sheffield, United Kingdom. 
      A.Stolzing@sheffield.ac.uk
FAU - Coleman, Natalie
AU  - Coleman N
FAU - Scutt, Andrew
AU  - Scutt A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Rejuvenation Res
JT  - Rejuvenation research
JID - 101213381
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cellular Senescence/*drug effects
MH  - Colony-Forming Units Assay
MH  - Female
MH  - Glucose/*pharmacology
MH  - Mesenchymal Stem Cells/cytology/*physiology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2006/04/13 09:00
MHDA- 2006/06/01 09:00
CRDT- 2006/04/13 09:00
PHST- 2006/04/13 09:00 [pubmed]
PHST- 2006/06/01 09:00 [medline]
PHST- 2006/04/13 09:00 [entrez]
AID - 10.1089/rej.2006.9.31 [doi]
PST - ppublish
SO  - Rejuvenation Res. 2006 Spring;9(1):31-5. doi: 10.1089/rej.2006.9.31.