PMID- 16610585
OWN - NLM
STAT- MEDLINE
DCOM- 20060504
LR  - 20061115
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 68
IP  - 3
DP  - 2006 Mar 2
TI  - Immunofluorescent test for simultaneous detection of Edwardsiella ictaluri and 
      Flavobacterium columnare.
PG  - 197-207
AB  - Enteric septicemia of catfish (ESC) and columnaris disease are 2 bacterial 
      diseases significantly affecting the aquaculture industry, and thus rapid 
      diagnosis of disease is imperative for making judicious management decisions. A 
      rapid indirect fluorescent antibody (IFA) test with antibody conjugated 
      fluorochromes having 2 different spectral properties (Alexa Fluor 488-emitting 
      green fluorescence, and Alexa Fluor 594-emitting red fluorescence) was compared 
      with bacteriological culture (accepted standard) for simultaneous detection of 
      Edwardsiella ictaluri (EI) and Flavobacterium columnare (FC) in 3 groups of 
      experimentally infected channel catfish (Ictalurus punctatus Rafinesque), and a 
      fourth group that acquired an aquarium-infection with F. columnare. A total of 
      303 samples (derived from kidney, brain and nares) from 101 fish were 
      concurrently examined by both tests. Fish in the 3 experimentally infected groups 
      (I to III) were culture positive for the bacteria with which they were infected, 
      and fish in Group IV, (the spontaneously infected fish) revealed F. columnare 
      only. The IFA test compared favorably in sensitivity (EI= 80.7 %; FC = 87.2%) and 
      specificity (EI = 83.9%; FC = 88.9%) with the standard bacteriological culture. 
      The positive predictive value (EI = 96.2% Group I, 90.8% Group II, 93.7% Groups I 
      and II combined; FC = 95.2% Group II, 95.3% Groups II, III and IV combined) was 
      high, while the negative predictive value (EI = 66.7% Group I, 31.3% Group II, 
      59.5% Groups I and II combined; FC = 73.7% Group II, 72.7% Groups II, III and IV 
      combined) was relatively low. The IFA test will serve as an efficient tool for 
      rapid simultaneous detection of E. ictaluri and F. columnare in outbreaks of 
      disease.
FAU - Panangalal, Victor S
AU  - Panangalal VS
AD  - Aquatic Animal Health Research Unit, US Department of Agriculture, Agricultural 
      Research Service, PO Box 952, Auburn, Alabama 36831, USA. vpanangala@ars.usda.gov
FAU - Shelby, Richard A
AU  - Shelby RA
FAU - Shoemaker, Craig A
AU  - Shoemaker CA
FAU - Klesius, Phillip H
AU  - Klesius PH
FAU - Mitra, Amitava
AU  - Mitra A
FAU - Morrison, Edward E
AU  - Morrison EE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
SB  - IM
MH  - Animals
MH  - Bacteriological Techniques/standards/veterinary
MH  - Edwardsiella ictaluri/*isolation & purification
MH  - Enterobacteriaceae Infections/*diagnosis
MH  - Fish Diseases/*diagnosis/microbiology
MH  - Fisheries/methods
MH  - Flavobacteriaceae Infections/*diagnosis
MH  - Flavobacterium/*isolation & purification
MH  - Fluorescent Antibody Technique, Indirect/standards/veterinary
MH  - *Ictaluridae
MH  - Immunoblotting/veterinary
MH  - Sensitivity and Specificity
EDAT- 2006/04/14 09:00
MHDA- 2006/05/05 09:00
CRDT- 2006/04/14 09:00
PHST- 2006/04/14 09:00 [pubmed]
PHST- 2006/05/05 09:00 [medline]
PHST- 2006/04/14 09:00 [entrez]
AID - 10.3354/dao068197 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2006 Mar 2;68(3):197-207. doi: 10.3354/dao068197.