PMID- 16615359 OWN - NLM STAT- MEDLINE DCOM- 20060531 LR - 20200314 IS - 0032-5791 (Print) IS - 0032-5791 (Linking) VI - 85 IP - 4 DP - 2006 Apr TI - Intestinal bacterial community and growth performance of chickens fed diets containing antibiotics. PG - 747-52 AB - This study was conducted to relate the performance of broiler chickens fed diets containing growth-promoting antibiotics to changes in the intestinal microbiota. The technique of denaturing gradient gel electrophoresis (DGGE) of amplicons of the region V3 of 16S rDNA was used to characterize the microbiota. Two experiments were conducted, one with broilers raised in battery cages and the other with broilers raised in floor pens. Antibiotics improved the performance of the chickens raised in floor pens only. Avilamycin, bacitracin methylene disalicylate, and enramycin induced changes in the composition of the intestinal bacterial community of the birds in both experiments. The number of bacterial genotypes found in the intestinal tract of chickens was not reduced by the antibiotics supplemented in either environment. However, the changes in the composition of the intestinal bacterial community induced by antibiotics may be related to improvement in growth performance. This was indicated by the suppression of 6 amplicons and the presence of 4 amplicons exclusive to the treatment that had the best performance in the floor pen experiment. FAU - Pedroso, A A AU - Pedroso AA AD - Department of Animal Science, College of Agriculture, University of Sao Paulo, 13418-900, Piracicaba, SP, Brazil. FAU - Menten, J F M AU - Menten JF FAU - Lambais, M R AU - Lambais MR FAU - Racanicci, A M C AU - Racanicci AM FAU - Longo, F A AU - Longo FA FAU - Sorbara, J O B AU - Sorbara JO LA - eng PT - Journal Article PT - Randomized Controlled Trial PT - Research Support, Non-U.S. Gov't PL - England TA - Poult Sci JT - Poultry science JID - 0401150 RN - 0 (Anti-Bacterial Agents) RN - 0 (DNA, Bacterial) RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Animal Feed/analysis MH - Animals MH - Anti-Bacterial Agents/*administration & dosage/analysis/*pharmacology MH - Bacteria/genetics/isolation & purification MH - Chickens/*growth & development/*microbiology MH - DNA, Bacterial/genetics MH - *Diet MH - Genetic Variation MH - Intestines/*drug effects/*microbiology MH - Male MH - Phylogeny MH - RNA, Ribosomal, 16S/genetics EDAT- 2006/04/18 09:00 MHDA- 2006/06/01 09:00 CRDT- 2006/04/18 09:00 PHST- 2006/04/18 09:00 [pubmed] PHST- 2006/06/01 09:00 [medline] PHST- 2006/04/18 09:00 [entrez] AID - S0032-5791(19)42178-0 [pii] AID - 10.1093/ps/85.4.747 [doi] PST - ppublish SO - Poult Sci. 2006 Apr;85(4):747-52. doi: 10.1093/ps/85.4.747.