PMID- 16615891
OWN - NLM
STAT- MEDLINE
DCOM- 20060516
LR  - 20131121
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 125
IP  - 1
DP  - 2006 Apr 7
TI  - An RNA sensor for intracellular Mg(2+).
PG  - 71-84
AB  - Most RNA molecules require Mg(2+) for their structure and enzymatic properties. 
      Here we report the first example of an RNA serving as sensor for cytoplasmic 
      Mg(2+). We establish that expression of the Mg(2+) transporter MgtA of Salmonella 
      enterica serovar Typhimurium is controlled by its 5' untranslated region (5'UTR). 
      We show that the 5'UTR of the mgtA gene can adopt different stem-loop structures 
      depending on the Mg(2+) levels, which determine whether transcription reads 
      through into the mgtA coding region or stops within the 5'UTR. We could 
      recapitulate the Mg(2+)-regulated transcription using a defined in vitro 
      transcription system with RNA polymerase as the only protein component. The 
      initiation of mgtA transcription responds to extracytoplasmic Mg(2+) and its 
      elongation into the coding region to cytoplasmic Mg(2+), providing a singular 
      example in which the same ligand is sensed in different cellular compartments to 
      regulate disparate steps in gene transcription.
FAU - Cromie, Michael J
AU  - Cromie MJ
AD  - Howard Hughes Medical Institute, Department of Molecular Microbiology, Washington 
      University School of Medicine, 660 South Euclid Avenue, Campus Box 8230, St. 
      Louis, MO 63110, USA.
FAU - Shi, Yixin
AU  - Shi Y
FAU - Latifi, Tammy
AU  - Latifi T
FAU - Groisman, Eduardo A
AU  - Groisman EA
LA  - eng
GR  - AI49561/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.- (Ribonucleases)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.- (MgtA protein, bacteria)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - 5' Untranslated Regions/*chemistry/genetics/metabolism
MH  - Adenosine Triphosphatases/genetics
MH  - Bacterial Proteins/genetics
MH  - Biosensing Techniques/*methods
MH  - Conserved Sequence/genetics
MH  - Cytoplasm/chemistry
MH  - Gene Expression Regulation, Bacterial
MH  - Magnesium/*analysis/*metabolism/pharmacology
MH  - Membrane Transport Proteins/genetics
MH  - Models, Genetic
MH  - Mutation/genetics
MH  - *Nucleic Acid Conformation/drug effects
MH  - Open Reading Frames/genetics
MH  - Phylogeny
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Bacterial/*chemistry/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Regulatory Sequences, Nucleic Acid/genetics
MH  - Ribonucleases/metabolism
MH  - Terminator Regions, Genetic/genetics
MH  - Transcription, Genetic/drug effects
EDAT- 2006/04/18 09:00
MHDA- 2006/05/17 09:00
CRDT- 2006/04/18 09:00
PHST- 2005/04/18 00:00 [received]
PHST- 2005/11/23 00:00 [revised]
PHST- 2006/01/05 00:00 [accepted]
PHST- 2006/04/18 09:00 [pubmed]
PHST- 2006/05/17 09:00 [medline]
PHST- 2006/04/18 09:00 [entrez]
AID - S0092-8674(06)00294-7 [pii]
AID - 10.1016/j.cell.2006.01.043 [doi]
PST - ppublish
SO  - Cell. 2006 Apr 7;125(1):71-84. doi: 10.1016/j.cell.2006.01.043.