PMID- 16616604
OWN - NLM
STAT- MEDLINE
DCOM- 20060711
LR  - 20151119
IS  - 0301-5629 (Print)
IS  - 0301-5629 (Linking)
VI  - 32
IP  - 4
DP  - 2006 Apr
TI  - Effects of low-intensity focused ultrasound on the mouse submandibular gland.
PG  - 587-94
AB  - Ultrasound is expected to make a considerable contribution to drug delivery 
      systems (DDSs). We tested the hypothesis that low-intensity focused ultrasound 
      (LIFU) increases vessel permeability in the mouse submandibular gland without 
      causing parenchymal damage. In a preliminary study, LIFU at 3 W/cm2 with a 50% 
      duty cycle for 2 minutes did not cause histologic damage. We therefore applied 
      LIFU to mouse submandibular gland at these conditions before and after injecting 
      horseradish peroxidase. Single labeling laser scanning confocal microscopy 
      revealed positive horseradish peroxidase staining around the excretory ducts in 
      the mucous-producing part of the gland, but absence of staining in control 
      glands. Immunostaining for fibrinogen was positive in the same region. Fibrinogen 
      is an intravascular protein that does not pass through intact vessels. These 
      findings suggest that LIFU increases vessel permeability and disruption without 
      destruction. It is anticipated that this process will be useful in establishing a 
      DDS that uses LIFU.
FAU - Shuto, Jun
AU  - Shuto J
AD  - Department of Otolaryngology, Oita University Faculty of Medicine, Yufu-city, 
      Oita, Japan.
FAU - Ichimiya, Issei
AU  - Ichimiya I
FAU - Suzuki, Masashi
AU  - Suzuki M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ultrasound Med Biol
JT  - Ultrasound in medicine & biology
JID - 0410553
RN  - 9001-32-5 (Fibrinogen)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Animals
MH  - Capillary Permeability
MH  - Drug Delivery Systems
MH  - Fibrinogen/metabolism
MH  - Horseradish Peroxidase
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Microscopy, Confocal
MH  - Submandibular Gland/blood supply/metabolism/*pathology
MH  - Ultrasonic Therapy/*adverse effects/methods
EDAT- 2006/04/18 09:00
MHDA- 2006/07/13 09:00
CRDT- 2006/04/18 09:00
PHST- 2005/06/27 00:00 [received]
PHST- 2005/11/03 00:00 [revised]
PHST- 2005/11/11 00:00 [accepted]
PHST- 2006/04/18 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2006/04/18 09:00 [entrez]
AID - S0301-5629(05)00464-3 [pii]
AID - 10.1016/j.ultrasmedbio.2005.11.014 [doi]
PST - ppublish
SO  - Ultrasound Med Biol. 2006 Apr;32(4):587-94. doi: 
      10.1016/j.ultrasmedbio.2005.11.014.