PMID- 16616824
OWN - NLM
STAT- MEDLINE
DCOM- 20070119
LR  - 20171116
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 42
IP  - 2
DP  - 2006 Sep 18
TI  - Determination of desoxyepothilone B in nude mice plasma by liquid-liquid 
      extraction and reversed-phase high-performance liquid chromatography.
PG  - 272-6
AB  - A novel reversed-phase high-performance liquid chromatographic (HPLC) method has 
      been established for the determination of a newly developed anti-cancer agent 
      desoxyepothilone B (dEpo B) in nude mice plasma. The sample preparation involved 
      deproteination of 200 microl of plasma sample first, followed by liquid-liquid 
      extraction of the resultant supernatant with chloroform. The compound taxol was 
      used as the internal standard. Chromatographic separations were carried out on a 
      250 mm x 4.6 mm Zorbax SB-phenyl column with acetonitrile-0.25% orthophosphoric 
      acid (50/50, v/v) as mobile phase and UV detection at 250 nm. For dEpo B and 
      taxol at the concentration level of 10 microg/ml in nude mice plasma, the 
      absolute extraction recoveries were 85.3 and 87.2%, respectively. The linear 
      quantification range of the method was 0.1-100 microg/ml in nude mice plasma with 
      linear correlation coefficients greater than 0.999. The within-day and 
      between-day relative standard deviations (R.S.D.s) for dEpo B at 0.5, 2.5 and 10 
      microg/ml levels in nude mice plasma fell in the range of 2.8-4.8 and 1.5-4.6%, 
      and the within-day and between-day recoveries were in the range of 96.5-101.7 and 
      97.7-101.2%, respectively.
FAU - Wang, Zhi
AU  - Wang Z
AD  - Department of Pharmacology and Toxicology, Division of Clinical Pharmacology, and 
      Comprehensive Cancer Center, University of Alabama at Birmingham, 35294-0019, 
      USA. wangzhi@mail.hebau.edu.cn
FAU - Wang, Hui
AU  - Wang H
FAU - Rhie, Julie K
AU  - Rhie JK
FAU - Covey, Joseph M
AU  - Covey JM
FAU - Liang, Ping
AU  - Liang P
FAU - Wang, Shuyi
AU  - Wang S
FAU - Wang, Chun
AU  - Wang C
FAU - Hu, Yanxue
AU  - Hu Y
FAU - Prasad, Gautam
AU  - Prasad G
FAU - Nan, Li
AU  - Nan L
FAU - Hill, Donald L
AU  - Hill DL
FAU - Zhang, Ruiwen
AU  - Zhang R
LA  - eng
GR  - N01-CM-07111/CM/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060417
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Epothilones)
RN  - T0358E0YUF (desoxyepothilone B)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*blood/pharmacokinetics
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Epothilones/*blood/pharmacokinetics
MH  - Mice
MH  - Mice, Nude
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2006/04/18 09:00
MHDA- 2007/01/20 09:00
CRDT- 2006/04/18 09:00
PHST- 2005/12/23 00:00 [received]
PHST- 2006/02/23 00:00 [revised]
PHST- 2006/02/28 00:00 [accepted]
PHST- 2006/04/18 09:00 [pubmed]
PHST- 2007/01/20 09:00 [medline]
PHST- 2006/04/18 09:00 [entrez]
AID - S0731-7085(06)00224-X [pii]
AID - 10.1016/j.jpba.2006.02.047 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2006 Sep 18;42(2):272-6. doi: 10.1016/j.jpba.2006.02.047. 
      Epub 2006 Apr 17.