PMID- 16617062
OWN - NLM
STAT- MEDLINE
DCOM- 20061003
LR  - 20220316
IS  - 0305-7453 (Print)
IS  - 0305-7453 (Linking)
VI  - 57
IP  - 6
DP  - 2006 Jun
TI  - High prevalence of type b beta-lactamase-non-producing ampicillin-resistant 
      Haemophilus influenzae in meningitis: the situation in Japan where Hib vaccine 
      has not been introduced.
PG  - 1077-82
AB  - OBJECTIVES: To study yearly changes in resistance and to identify ftsI mutations 
      in beta-lactamase-non-producing ampicillin-resistant (BLNAR) and TEM-1 
      beta-lactamase-producing amoxicillin/clavulanic acid-resistant (BLPACR) isolates 
      of Haemophilus influenzae from patients with meningitis. METHODS: Between January 
      2000 and December 2004, we received 621 isolates of H. influenzae from 285 member 
      institutions of the Nationwide Surveillance Study Group for Bacterial Meningitis. 
      All isolates were analysed by PCR to identify resistance genes and tested for 
      susceptibility to beta-lactams. The ftsI gene was sequenced in all BLNAR and 
      BLPACR isolates. RESULTS: All but four isolates were of serotype b. The isolates 
      could be divided into six classes, namely beta-lactamase-non-producing 
      ampicillin-susceptible (25.0%), TEM-1 beta-lactamase-producing 
      ampicillin-resistant (11.0%), beta-lactamase-non-producing low-level 
      ampicillin-resistant with N526K or R517H substitution in the ftsI gene (30.4%), 
      BLNAR with an S385T substitution together with either N526K or R517H substitution 
      in ftsI (22.2%), BLPACR-I with either a N526K or R517H substitution in ftsI 
      (9.5%) and BLPACR-II with an S385T substitution together with either a N526K or 
      R517H substitution in ftsI (1.9%). The prevalence of BLNAR has increased rapidly, 
      from 5.8% in 2000 to 34.5% in 2004. All BLNAR and BLPACR-II strains were 
      classified into nine subgroups on the basis of substitution patterns in the ftsI 
      gene. The MICs of cephalosporin antibiotics for H. influenzae transformants into 
      which the ftsI genes from BLNAR strains of each of the nine subgroups were 
      introduced increased to varying degrees depending on the mutations. CONCLUSIONS: 
      The results suggest that introduction of H. influenzae type b (Hib) vaccination 
      into infants and children is necessary for the prevention of severe Hib 
      infections in Japan.
FAU - Hasegawa, Keiko
AU  - Hasegawa K
AD  - Laboratory of Infectious Agents Surveillance, Kitasato Institute for Life 
      Sciences, Kitasato University 5-9-1, Shirokane, Minato-ku, Tokyo, Japan.
FAU - Kobayashi, Reiko
AU  - Kobayashi R
FAU - Takada, Emi
AU  - Takada E
FAU - Ono, Akiko
AU  - Ono A
FAU - Chiba, Naoko
AU  - Chiba N
FAU - Morozumi, Miyuki
AU  - Morozumi M
FAU - Iwata, Satoshi
AU  - Iwata S
FAU - Sunakawa, Keisuke
AU  - Sunakawa K
FAU - Ubukata, Kimiko
AU  - Ubukata K
CN  - Nationwide Surveillance for Bacterial Meningitis
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060414
PL  - England
TA  - J Antimicrob Chemother
JT  - The Journal of antimicrobial chemotherapy
JID - 7513617
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Penicillin-Binding Proteins)
RN  - 0 (beta-Lactams)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - EC 3.5.2.6 (beta-lactamase TEM-1)
SB  - IM
MH  - Amino Acid Substitution
MH  - Ampicillin Resistance/*genetics
MH  - Anti-Bacterial Agents/pharmacology
MH  - Child, Preschool
MH  - DNA, Bacterial/analysis/chemistry/genetics
MH  - Gene Transfer, Horizontal
MH  - Genes, Bacterial
MH  - Haemophilus influenzae type b/*drug effects/enzymology/genetics/isolation & 
      purification
MH  - Humans
MH  - Infant
MH  - Japan
MH  - Meningitis, Haemophilus/*microbiology
MH  - Microbial Sensitivity Tests
MH  - Mutation
MH  - Mutation, Missense
MH  - Penicillin-Binding Proteins/genetics
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Transformation, Bacterial
MH  - beta-Lactamases/*analysis
MH  - beta-Lactams/pharmacology
EDAT- 2006/04/18 09:00
MHDA- 2006/10/04 09:00
CRDT- 2006/04/18 09:00
PHST- 2006/04/18 09:00 [pubmed]
PHST- 2006/10/04 09:00 [medline]
PHST- 2006/04/18 09:00 [entrez]
AID - dkl142 [pii]
AID - 10.1093/jac/dkl142 [doi]
PST - ppublish
SO  - J Antimicrob Chemother. 2006 Jun;57(6):1077-82. doi: 10.1093/jac/dkl142. Epub 
      2006 Apr 14.