PMID- 16617361
OWN - NLM
STAT- MEDLINE
DCOM- 20140320
LR  - 20171116
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 38
IP  - 2
DP  - 2006 Apr 18
TI  - Apoptosis of human hepatoma cell lines induced by transforming growth factor beta 
      1 (TGF-beta1) correlates with p53 and Smad4 activation.
PG  - 176-8
AB  - OBJECTIVE: To determine the relationships between apoptosis induced by 
      transforming growth factor beta 1(TGF-beta1) and Smad in human hepatoma cell 
      lines. METHODS: Three human hepatic carcinoma cell lines, involving different 
      status of the p53 gene respectively, were used in this study. TGF-beta1-induced 
      apoptosis in hepatic carcinoma cell lines was quantitated using the terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. For 
      identification of the mechanism of apoptosis induced by TGF-beta1, these cell 
      lines were transfected with a TGF-beta1-inducible luciferase reporter plasmid 
      containing Smad binding elements (SBE) and luciferase gene using LF2000, then 
      were treated with TGF-beta1. Relative luciferase activity was assayed 
      respectively. RESULTS: Among three cell lines studied with TUNEL assay, addition 
      of TGF-beta1 induced apoptosis only in HepG2 cells (wild type p53). In contrast, 
      Huh-7 (mutant p53) and Hep3B (deleted p53) cell lines lacked apoptosis. The 
      detection of luciferase activity indicated that HepG2 cells dramatically 
      increased the response to TGF-beta1 induction, Huh-7 and Hep3B cell lines 
      significantly lowered luciferase expression. CONCLUSION: HepG2 cells were highly 
      susceptible to TGF-beta1-induced apoptosis compared with Hep3B and Huh-7 cell 
      lines. Smad4 may be a central mediator of the TGF-beta1 signaling transduction 
      pathway.
FAU - Wang, Chun-lei
AU  - Wang CL
AD  - Department of General Surgery, Peking University First Hospital, Beijing 100034, 
      China. wangchunlei301@yahoo.com
FAU - Wan, Yuan-lian
AU  - Wan YL
FAU - Liu, Yu-cun
AU  - Liu YC
FAU - Huang, Zhi-qiang
AU  - Huang ZQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
RN  - 0 (SMAD4 protein, human)
RN  - 0 (Smad4 Protein)
RN  - 0 (TP53 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Hepatocellular/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Hep G2 Cells
MH  - Humans
MH  - Signal Transduction/drug effects
MH  - Smad4 Protein/*metabolism
MH  - Transforming Growth Factor beta1/*pharmacology
MH  - Tumor Suppressor Protein p53/*metabolism
EDAT- 2006/04/18 09:00
MHDA- 2014/03/22 06:00
CRDT- 2006/04/18 09:00
PHST- 2006/04/18 09:00 [pubmed]
PHST- 2014/03/22 06:00 [medline]
PHST- 2006/04/18 09:00 [entrez]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Apr 18;38(2):176-8.