PMID- 16618365
OWN - NLM
STAT- MEDLINE
DCOM- 20070326
LR  - 20171116
IS  - 0905-6157 (Print)
IS  - 0905-6157 (Linking)
VI  - 17
IP  - 2
DP  - 2006 Mar
TI  - Basophil Activation Test and specific IgE measurements using a panel of 
      recombinant natural rubber latex allergens to determine the latex allergen 
      sensitization profile in children.
PG  - 148-56
AB  - There are no documented studies that describe natural rubber latex (NRL) 
      sensitization in children with a history of surgical intervention but without any 
      congenital malformation (urogenital anomalies, spina bifida, etc.), although some 
      authors have studied NRL allergy in children without a history of surgical 
      intervention. The aim of this work was to evaluate the sensitization profile to 
      single NRL allergens in children without spina bifida and without repeated 
      surgical interventions, by using different recombinant and natural latex 
      allergens in two analytical techniques: specific serum immunoglobulin E (IgE) 
      quantification and flow cytometry determination of activated basophils expressing 
      CD63, after stimulating cells from patients with NRL allergens. A total of 23 
      patients and 10 healthy children were selected. Conjunctival and in-use NRL 
      provocation tests were carried out, as well as specific IgE determination in all 
      patients' and controls' sera with the recombinant NRL allergens: rHev b 1, rHev b 
      2, rHev b 3, rHev b 5, rHev b 6.01, rHev b 6.02, rHev b 8, rHev b 9 and rHev b 11 
      and with NRL (k82) using appropriate ImmunoCAPs. The Basophil Activation Test 
      (BAT) was performed with whole latex extract and with the recombinant allergens 
      rHev b 5 and rHev b 6.01, as well as with the natural allergen Hev b 6.02. The 
      sensitivity and the specificity of NRL-specific IgE (k82) were 100%. Positive IgE 
      responses to rHev b 5 were found in sera of 10 children, to rHev b 6.01 in 16 and 
      for rHev b 6.02 in 15 children's sera. Specific IgE to rHev b 8 was found in four 
      sera of the children. We only found significant differences in sensitization to 
      rHev b 5 in children with two or more surgical interventions compared with the 
      non-intervened group or those with only one intervention. Specific IgE in sera of 
      children with latex-fruit syndrome recognized rHev b 6.02, but not to rHev b 11. 
      The patients sensitized to Hev b 8, Hev b 9 and/or Hev b 11 were atopic. The four 
      patients presenting a positive response to the NRL profilin Hev b 8 were allergic 
      to pollen. The BAT against whole NRL extract was positive in 22 of 23 children; 
      against rHev b 5 in 14 of the patients studied; against rHev b 6.01 in seven 
      cases and against nHev b 6.02 in 19 children. In all the control subjects, the 
      results using this technique were negative. If combined rHev b 5, rHev b 6.01 and 
      nHev b 6.02 together, BAT could detect 20 of the 23 children with latex allergy. 
      The combined use of ImmunoCAP with all the recombinant NRL allergens and BAT with 
      rHev b 5, rHev b 6.01 and nHev b 6.02, enabled the identification of NRL allergy 
      in 22 of 23 patients. There is a positive and significant correlation between 
      sensitization to Hev b 5 and the number of interventions. BAT and 
      allergen-specific IgE determination could be used as first-line in vitro 
      diagnostic tests in patients with NRL allergy.
FAU - Sanz, Maria L
AU  - Sanz ML
AD  - Department of Allergology and Clinical Immunology, Clinica Universitaria, 
      Universidad de Navarra, Pamplona, Spain. mlsanzar@unav.es
FAU - Garcia-Aviles, M C
AU  - Garcia-Aviles MC
FAU - Tabar, Ana I
AU  - Tabar AI
FAU - Anda, M
AU  - Anda M
FAU - Garcia, B E
AU  - Garcia BE
FAU - Barber, Domingo
AU  - Barber D
FAU - Salcedo, Gabriel
AU  - Salcedo G
FAU - Rihs, Hans-Peter
AU  - Rihs HP
FAU - Raulf-Heimsoth, Monika
AU  - Raulf-Heimsoth M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (Allergens)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Plant)
RN  - 0 (CD63 protein, human)
RN  - 0 (Plant Proteins)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tetraspanin 30)
RN  - 0 (allergen Hev b 5)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-04-6 (Rubber)
SB  - IM
MH  - Adolescent
MH  - Allergens/*immunology
MH  - Antibody Specificity
MH  - Antigens, CD/biosynthesis
MH  - Antigens, Plant
MH  - *Basophil Degranulation Test
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoglobulin E/*blood/immunology
MH  - Latex Hypersensitivity/diagnosis/*immunology
MH  - Male
MH  - Plant Proteins/immunology
MH  - Platelet Membrane Glycoproteins/biosynthesis
MH  - Recombinant Proteins/immunology
MH  - Rubber
MH  - Tetraspanin 30
EDAT- 2006/04/19 09:00
MHDA- 2007/03/27 09:00
CRDT- 2006/04/19 09:00
PHST- 2006/04/19 09:00 [pubmed]
PHST- 2007/03/27 09:00 [medline]
PHST- 2006/04/19 09:00 [entrez]
AID - PAI359 [pii]
AID - 10.1111/j.1399-3038.2005.00359.x [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 2006 Mar;17(2):148-56. doi: 
      10.1111/j.1399-3038.2005.00359.x.