PMID- 16620893
OWN - NLM
STAT- MEDLINE
DCOM- 20060925
LR  - 20191210
IS  - 0039-128X (Print)
IS  - 0039-128X (Linking)
VI  - 71
IP  - 7
DP  - 2006 Jul
TI  - Studies on neurosteroids XVIII LC-MS analysis of changes in rat brain and serum 
      testosterone levels induced by immobilization stress and ethanol administration.
PG  - 609-17
AB  - The liquid chromatography-mass spectrometry (LC-MS) methods were developed and 
      validated for the determination of testosterone (T) in the brain and serum of 
      rats and of 5alpha-androstane-3alpha,17beta-diol (ADIOL), a metabolite of T, in 
      the brain of rats. After derivatization of T with 2-hydrazino-1-methylpyridine 
      and of ADIOL with p-nitrobenzoyl chloride, the detection sensitivities of T and 
      ADIOL using LC-MS were increased 70- and 400-times superior to those of intact T 
      and intact ADIOL, respectively. Those LC-MS methods are specific and reliable for 
      the analysis of trace amounts of T and ADIOL in small amounts of samples. The 
      animal studies using the developed methods showed that the brain and serum levels 
      of T and the brain levels of ADIOL were not changed by stress or ethanol 
      administration but the concentration ratio of the brain T to serum T in the 
      stressed rats was higher than that in untreated rats. The low levels of 
      endogenous AIDOL in brain of stressed and unrestrained rats found in this study 
      demonstrated that the contribution to anesthetic and anxiolytic effects of ADIOL 
      via gamma-aminobutyric acid type A receptors may be negligible.
FAU - Higashi, Tatsuya
AU  - Higashi T
AD  - Division of Pharmaceutical Sciences, Graduate School of Natural Science and 
      Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. 
      higashi@p.kanazawa-u.ac.jp
FAU - Ninomiya, Yasuaki
AU  - Ninomiya Y
FAU - Iwaki, Naoko
AU  - Iwaki N
FAU - Yamauchi, Akinori
AU  - Yamauchi A
FAU - Takayama, Naoki
AU  - Takayama N
FAU - Shimada, Kazutake
AU  - Shimada K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20060418
PL  - United States
TA  - Steroids
JT  - Steroids
JID - 0404536
RN  - 0 (2-hydrazino-1-methylpyridine)
RN  - 0 (Hydrazines)
RN  - 0 (Nitrobenzoates)
RN  - 0 (Pyridines)
RN  - 25126-76-5 (Androstane-3,17-diol)
RN  - 3K9958V90M (Ethanol)
RN  - 3XMK78S47O (Testosterone)
RN  - X3H8PW2GC4 (4-nitrobenzoyl chloride)
SB  - IM
MH  - Androstane-3,17-diol/blood/*metabolism
MH  - Animals
MH  - Brain/cytology/drug effects/*metabolism
MH  - Chromatography, Liquid/*methods
MH  - Ethanol/pharmacology
MH  - Hydrazines/chemistry
MH  - Male
MH  - Mass Spectrometry/*methods
MH  - Nitrobenzoates/chemistry
MH  - Pyridines/chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Restraint, Physical
MH  - Stress, Physiological/*metabolism
MH  - Testosterone/blood/*metabolism
EDAT- 2006/04/20 09:00
MHDA- 2006/09/26 09:00
CRDT- 2006/04/20 09:00
PHST- 2005/12/14 00:00 [received]
PHST- 2006/02/15 00:00 [revised]
PHST- 2006/03/14 00:00 [accepted]
PHST- 2006/04/20 09:00 [pubmed]
PHST- 2006/09/26 09:00 [medline]
PHST- 2006/04/20 09:00 [entrez]
AID - S0039-128X(06)00065-1 [pii]
AID - 10.1016/j.steroids.2006.03.003 [doi]
PST - ppublish
SO  - Steroids. 2006 Jul;71(7):609-17. doi: 10.1016/j.steroids.2006.03.003. Epub 2006 
      Apr 18.