PMID- 16621159
OWN - NLM
STAT- MEDLINE
DCOM- 20061002
LR  - 20191210
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 49
IP  - 4
DP  - 2006 Sep
TI  - Modulation of NMDA receptors by AKT kinase.
PG  - 351-8
AB  - The aim of the present work was to assess whether Akt modulates NMDA receptor 
      function in cerebellar neurons in culture. Forskolin increases cAMP and activates 
      Akt and NMDA receptors. In neurons treated with forskolin, intracellular calcium 
      increased to 296 +/- 38% and this was completely prevented by inhibition of Akt. 
      This indicates that, in these neurons, cAMP modulates NMDA receptors via Epac and 
      Akt. Brain derived neurotrophic factor (BDNF) increases phosphorylation (and 
      activity) of Akt to 350 +/- 60% of basal and also potentiates the increase of 
      calcium and in cGMP induced by NMDA. BDNF-induced potentiation of NMDA receptor 
      function is completely prevented by inhibition of PI3 kinase or of Akt. This 
      indicates that BDNF modulates NMDA receptor function via PI3 kinase and Akt. 
      Activation of NMDA receptors also leads to phosphorylation and activation of Akt 
      which, in turn, potentiates NMDA receptor activation. The results reported 
      indicate that when Akt activity increases the activation of NMDA receptors by its 
      agonists also increases. Akt may play important roles in the modulation of NMDA 
      receptor responses by other neurotransmitters and modulators and in the 
      adaptation of NMDA receptor function to the physiological environmental 
      conditions.
FAU - Sanchez-Perez, Ana Maria
AU  - Sanchez-Perez AM
AD  - Laboratory of Neurobiology, Centro de Investigacion Principe Felipe, Valencia, 
      Spain.
FAU - Llansola, Marta
AU  - Llansola M
FAU - Felipo, Vicente
AU  - Felipo V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060418
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 1F7A44V6OU (Colforsin)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - H2D2X058MU (Cyclic GMP)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Calcium/metabolism
MH  - Cells, Cultured
MH  - Cerebellum/cytology/drug effects/enzymology
MH  - Colforsin/pharmacology
MH  - Cyclic GMP/chemistry/metabolism
MH  - Drug Synergism
MH  - Enzyme Activation/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Immunoblotting
MH  - Immunoprecipitation
MH  - N-Methylaspartate/pharmacology
MH  - Neurons/drug effects/enzymology/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/antagonists & inhibitors/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/*drug effects/metabolism
EDAT- 2006/04/20 09:00
MHDA- 2006/10/03 09:00
CRDT- 2006/04/20 09:00
PHST- 2005/10/18 00:00 [received]
PHST- 2006/02/01 00:00 [revised]
PHST- 2006/02/20 00:00 [accepted]
PHST- 2006/04/20 09:00 [pubmed]
PHST- 2006/10/03 09:00 [medline]
PHST- 2006/04/20 09:00 [entrez]
AID - S0197-0186(06)00097-0 [pii]
AID - 10.1016/j.neuint.2006.02.005 [doi]
PST - ppublish
SO  - Neurochem Int. 2006 Sep;49(4):351-8. doi: 10.1016/j.neuint.2006.02.005. Epub 2006 
      Apr 18.