PMID- 1662389
OWN - NLM
STAT- MEDLINE
DCOM- 19920207
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 88
IP  - 24
DP  - 1991 Dec 15
TI  - Human immunodeficiency virus glycoprotein (gp120) infused into rat brain induces 
      interleukin 1 to elevate pituitary-adrenal activity and decrease peripheral 
      cellular immune responses.
PG  - 11246-50
AB  - Intracerebroventricular (i.c.v.) infusion of glycosylated recombinant gp120, the 
      envelope protein of human immunodeficiency virus, in various doses (100 ng to 4 
      micrograms) resulted in detection of interleukin 1 (IL-1) activity in a high 
      percentage (61%; 33 of 54) of rat brains, whereas IL-1 was very rarely detected 
      in brains of animals infused with several control substances (4%; 1 of 28). To 
      detect IL-1, clarified glial lysate of diencephalon plus brainstem was subjected 
      to gel exclusion chromatography and fractions were assessed for thymocyte 
      stimulation. IL-1 was seen 2, 6, and 24 hr postinfusion. i.c.v. gp120 also 
      produced known effects of IL-1 in brain, elevating steroid concentration in 
      plasma and decreasing cellular immune responses [natural killer (NK) cell 
      activity and mitogenic response to Con A] of blood and splenic lymphocytes. When 
      gp120 was infused together with alpha-melanocyte-stimulating hormone (20 ng), 
      which blocks many biological actions of IL-1, gp120 no longer elevated steroids 
      or decreased NK cell activity. After intravenous gp120, IL-1 was not found in 
      brain or plasma, indicating that stimulation of IL-1 in brain by i.c.v. gp120 was 
      not due to gp120 affecting infiltrating cells from blood or to elevated 
      circulating IL-1. That induction of IL-1 in brain might have resulted from 
      lipopolysaccharide (LPS) in the gp120 solution was ruled out by studies showing 
      that (i) heating of the infusion solution, which does not affect the capacity of 
      LPS to induce IL-1, eliminated the ability of gp120 infusion to induce brain 
      IL-1, and (ii) gp120 induced IL-1 in brains of LPS-resistant C3H/HeJ mice. 
      Injection of gp120 directly into the hippocampus stimulated IL-1 more readily 
      than i.c.v. infusion. Thymocyte stimulation produced by active fractions of 
      gp120-infused brains was blocked by monoclonal antibody to IL-1 receptors. These 
      findings indicate that elevation of IL-1 in brain can result from infection with 
      human immunodeficiency virus and may be responsible for certain abnormalities 
      (e.g., elevated activity of pituitary-adrenal axis) seen in AIDS patients.
FAU - Sundar, S K
AU  - Sundar SK
AD  - Department of Psychiatry, Duke University Medical Center, Durham, NC 27710.
FAU - Cierpial, M A
AU  - Cierpial MA
FAU - Kamaraju, L S
AU  - Kamaraju LS
FAU - Long, S
AU  - Long S
FAU - Hsieh, S
AU  - Hsieh S
FAU - Lorenz, C
AU  - Lorenz C
FAU - Aaron, M
AU  - Aaron M
FAU - Ritchie, J C
AU  - Ritchie JC
FAU - Weiss, J M
AU  - Weiss JM
LA  - eng
GR  - MH45675/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Interleukin-1)
RN  - 581-05-5 (alpha-MSH)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/*physiology
MH  - Cerebral Ventricles/drug effects/*physiology
MH  - Corticosterone/*blood
MH  - HIV Envelope Protein gp120/*administration & dosage/toxicity
MH  - Immunity, Cellular/*drug effects
MH  - Infusions, Intravenous
MH  - Infusions, Parenteral
MH  - Interleukin-1/biosynthesis/*physiology
MH  - Lymphocyte Activation
MH  - Male
MH  - Pituitary-Adrenal System/drug effects/*physiology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Reference Values
MH  - T-Lymphocytes/drug effects/*immunology
MH  - alpha-MSH/administration & dosage/*pharmacology
PMC - PMC53111
EDAT- 1991/12/15 00:00
MHDA- 1991/12/15 00:01
PMCR- 1992/06/15
CRDT- 1991/12/15 00:00
PHST- 1991/12/15 00:00 [pubmed]
PHST- 1991/12/15 00:01 [medline]
PHST- 1991/12/15 00:00 [entrez]
PHST- 1992/06/15 00:00 [pmc-release]
AID - 10.1073/pnas.88.24.11246 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11246-50. doi: 
      10.1073/pnas.88.24.11246.