PMID- 16624062
OWN - NLM
STAT- MEDLINE
DCOM- 20100528
LR  - 20181201
IS  - 0578-1310 (Print)
IS  - 0578-1310 (Linking)
VI  - 44
IP  - 3
DP  - 2006 Mar
TI  - [Differentiation of human umbilical cord blood mesenchymal stem cells toward 
      neurons induced by baicalin in vitro].
PG  - 214-9
AB  - OBJECTIVE: To study the possibility and mechanism by which the human umbilical 
      blood mesenchymal stem cells (MSCs) are induced to differentiate into neuron in 
      vitro by baicalin, a kind of flavonoid isolated from an important medicinal plant 
      Scutellariae Radix. METHODS: Human cord blood was obtained via sterile procedure 
      with 20 U/ml of preservative-free heparin. MSCs were isolated by the two-step 
      assay of gelatin sedimentation plus Ficoll centrifugation separation, purified 
      and amplified in the liquid culture medium. According to the kind of 
      antioxidants, the experiment was conducted in five groups: induction group, 
      control group I, control group II, control group III and control group IV. Five 
      subgroups of MSCs amplificated ex vivo for 2 weeks in each group were induced by 
      the media containing baicalin (BC, 200 - 400 micromol/L) or baicalin-free for 
      seven days. The media consisted of induction medium (DMEM plus 200 - 400 
      micromol/L of baicalin) and post-induction medium (DMEM plus 200 - 400 micromol/L 
      of baicalin, B27). The expression of neuronal or glia specific markers was 
      evaluated by using indirect immunofluorescence cytochemistry staining. The 
      percentage of differentiated cells and living cells was measured by Hoechest 
      33,258 staining assay. RESULTS: After induction for 7 days, MSCs displayed 
      neuronal morphologies, such as pyramidal cell bodies and processes formed 
      extensive network. The undifferentiated cells did not exhibit a neuronal or glial 
      phenotype, while the differentiating cells expressed NSE and MAP-2, the specific 
      markers of neuron, but did not express GFAP, the specific marker of glia on the 
      seventh day after induced by baicalin. The percentage of NSE and MAP-2 expressed 
      on the seventh day after induced by baicalin was (76.3 +/- 9.2)% and (78.5 +/- 
      5.5)%, respectively, which was significantly higher compared with control group 
      I, control group II and control group III (P < 0.01), respectively. In addition, 
      the ratio of living cells after induced for seven days in the BC group was (85.3 
      +/- 4.8)%, which increased significantly compared with control group I, control 
      group II and control group III (P < 0.01), respectively. CONCLUSIONS: Baicalin 
      may induce the umbilical blood MSCs to neuron or neuron-alike cells in vitro in a 
      moderate and stable manner. The mechanism of such an induction may be related 
      with its controlling the activity of NF-kappaB which regulates the production of 
      many kinds of cytokines, such as brain-derived neurotrophic factor (BDNF), etc.
FAU - Yan, Xiao-hua
AU  - Yan XH
AD  - Department of Pediatrics and Hematology, The First Affiliated Hospital, Nanchang 
      University, Nanchang 330006, China.
FAU - Huang, Rui-bin
AU  - Huang RB
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Ke Za Zhi
JT  - Zhonghua er ke za zhi = Chinese journal of pediatrics
JID - 0417427
RN  - 0 (Flavonoids)
RN  - 347Q89U4M5 (baicalin)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Differentiation/*drug effects
MH  - Cell Separation
MH  - Fetal Blood/*cytology
MH  - Flavonoids/*pharmacology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*drug effects
MH  - Neurons/*cytology
MH  - Staining and Labeling
EDAT- 2006/04/21 09:00
MHDA- 2010/05/29 06:00
CRDT- 2006/04/21 09:00
PHST- 2006/04/21 09:00 [pubmed]
PHST- 2010/05/29 06:00 [medline]
PHST- 2006/04/21 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Ke Za Zhi. 2006 Mar;44(3):214-9.