PMID- 16624383
OWN - NLM
STAT- MEDLINE
DCOM- 20060905
LR  - 20191210
IS  - 0048-9697 (Print)
IS  - 0048-9697 (Linking)
VI  - 366
IP  - 1
DP  - 2006 Jul 31
TI  - Evaluation of total lipids using enzymatic methods for the normalization of 
      persistent organic pollutant levels in serum.
PG  - 361-6
AB  - The human exposure to persistent organic pollutants (POPs) is conveniently 
      assessed through analysis of blood serum or plasma and the POPs concentrations 
      are traditionally expressed on a lipid weight basis. Enzymatic determinations of 
      total lipid (TL) content in serum, which imply the measurement of individual 
      lipid classes, such as triglycerides (TG), cholesterol (CHOL) and phospholipid 
      (PL), use small amounts of serum/plasma (<100 microl). These have also become a 
      more precise and less laborious alternative for the gravimetric lipid 
      determinations which were preferred in the past. However, PL are not routinely 
      measured in most clinical laboratories and therefore, TL values are often 
      predicted from formulas based on measurements of only TG and CHOL. In the present 
      study, a large data set (n=483) from Belgian, Swedish and Norwegian populations 
      was used to compute a new formula for TL [TL=1.33*TG+1.12*CHOL+1.48 (g/l)] by 
      means of multiple linear regression. A very good correlation (r=0.949, p<0.001) 
      was found between TL values calculated with the proposed formula and TL values 
      obtained by summation of TG, CHOL and PL. Statistically different values of TL 
      were computed when different previously published formulas were compared, which 
      can have a strong influence on the calculation of lipid-normalized concentrations 
      of POPs. Nevertheless, TL values derived using a mathematical formula which 
      includes only TG and CHOL will be less accurate than TL values obtained by 
      summation of TG, CHOL and PL, which should be the calculation method of choice if 
      PL measurements are available.
FAU - Covaci, Adrian
AU  - Covaci A
AD  - Toxicological Center, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, 
      Belgium. adrain.covaci@ua.ac.be
FAU - Voorspoels, Stefan
AU  - Voorspoels S
FAU - Thomsen, Cathrine
AU  - Thomsen C
FAU - van Bavel, Bert
AU  - van Bavel B
FAU - Neels, Hugo
AU  - Neels H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060419
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Environmental Pollutants)
RN  - 0 (Lipids)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Belgium
MH  - Biological Assay
MH  - Cholesterol/blood
MH  - Environmental Pollutants/*blood
MH  - Humans
MH  - Lipids/*blood
MH  - Middle Aged
MH  - Sweden
MH  - Triglycerides/blood
EDAT- 2006/04/21 09:00
MHDA- 2006/09/06 09:00
CRDT- 2006/04/21 09:00
PHST- 2006/01/16 00:00 [received]
PHST- 2006/03/03 00:00 [revised]
PHST- 2006/03/06 00:00 [accepted]
PHST- 2006/04/21 09:00 [pubmed]
PHST- 2006/09/06 09:00 [medline]
PHST- 2006/04/21 09:00 [entrez]
AID - S0048-9697(06)00237-3 [pii]
AID - 10.1016/j.scitotenv.2006.03.006 [doi]
PST - ppublish
SO  - Sci Total Environ. 2006 Jul 31;366(1):361-6. doi: 
      10.1016/j.scitotenv.2006.03.006. Epub 2006 Apr 19.