PMID- 16624570
OWN - NLM
STAT- MEDLINE
DCOM- 20060830
LR  - 20131121
IS  - 1011-1344 (Print)
IS  - 1011-1344 (Linking)
VI  - 84
IP  - 2
DP  - 2006 Aug 1
TI  - Effects of HeNe laser irradiation on experimental paracoccidioidomycotic lesions.
PG  - 141-9
AB  - Paracoccidioidomycosis (PCM) is the most prevalent human mycosis in Latin 
      America. The infection is thought to take place firstly in the lungs and then may 
      disseminate to other organs and tissues. Treatment by currently available 
      antifungals is lengthy, the drugs may have undesirable side effects, and some are 
      costly. Occasional resistant strains of Paracoccidioides brasiliensis, the 
      causative agent of PCM, have been reported. So, the search for more efficient 
      treatments or adjuvant therapies has to be continued. In this work, we evaluated 
      the effects of HeNe laser irradiation on cutaneous inflammatory lesions caused by 
      the inoculation of 5 x 10(6)/0.1ml yeasts cells into the back footpad of Balb/c 
      mice. HeNe irradiation (lambda=632.8nm, 3mW, incident energy of 3J/cm(2)) was 
      applied at days 7, 8 and 9 post-infection and histological and 
      immunohistochemical analysis were done. Unirradiated animals were used as 
      controls. The results showed that laser-treated mice presented reduction of 
      footpad edema, faster cutaneous wound healing, confluent granuloma, diffuse- and 
      more loosely distributed immunolabeling for TNF-alpha, enhanced labeling of 
      IFN-gamma and any P. brasiliensis form detected, whereas multiple viable fungi 
      were seen in diffuse widespread granulomas obtained from non-treated mice 
      foot-pad. Fungi that were harvested from laser-treated animals presented no 
      capability of growth in vitro as compared to those obtained from non-treated 
      mice. We conclude that HeNe laser irradiation was able to inhibit the progress of 
      inflammatory local reaction produced by P. brasiliensis infection and influence 
      local cytokines production. We suggest that this treatment modality can be a 
      useful coadjuvant tool to be combined with antifungal agents in the treatment of 
      PCM ulcerations. The mechanisms involved in laser therapy of PCM lesions need 
      further investigation.
FAU - Ferreira, Maria Carolina
AU  - Ferreira MC
AD  - Department of Microbiology and Immunology, Institute of Biology, State University 
      at Campinas (UNICAMP), Caixa Postal 6109, 13083-970 Campinas, Sao Paulo, Brazil.
FAU - Brito, Vania Nieto
AU  - Brito VN
FAU - Gameiro, Jacy
AU  - Gameiro J
FAU - Costa, Maria Renata Salles Nogueira
AU  - Costa MR
FAU - Vasconcellos, Elza Costa Cruz
AU  - Vasconcellos EC
FAU - Cruz-Hofling, Maria Alice
AU  - Cruz-Hofling MA
FAU - Verinaud, Liana
AU  - Verinaud L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060418
PL  - Switzerland
TA  - J Photochem Photobiol B
JT  - Journal of photochemistry and photobiology. B, Biology
JID - 8804966
RN  - 206GF3GB41 (Helium)
RN  - 4VB4Y46AHD (Neon)
SB  - IM
MH  - Animals
MH  - Helium
MH  - Immunohistochemistry
MH  - *Lasers
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neon
MH  - Paracoccidioidomycosis/microbiology/pathology/*therapy
EDAT- 2006/04/21 09:00
MHDA- 2006/08/31 09:00
CRDT- 2006/04/21 09:00
PHST- 2005/12/16 00:00 [received]
PHST- 2006/02/27 00:00 [revised]
PHST- 2006/02/28 00:00 [accepted]
PHST- 2006/04/21 09:00 [pubmed]
PHST- 2006/08/31 09:00 [medline]
PHST- 2006/04/21 09:00 [entrez]
AID - S1011-1344(06)00074-1 [pii]
AID - 10.1016/j.jphotobiol.2006.02.011 [doi]
PST - ppublish
SO  - J Photochem Photobiol B. 2006 Aug 1;84(2):141-9. doi: 
      10.1016/j.jphotobiol.2006.02.011. Epub 2006 Apr 18.