PMID- 16625101
OWN - NLM
STAT- MEDLINE
DCOM- 20060518
LR  - 20190823
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 30
IP  - 4
DP  - 2006 Apr
TI  - Molecular cytogenetic evidence of t(14;18)(IGH;BCL2) in a substantial proportion 
      of primary cutaneous follicle center lymphomas.
PG  - 529-36
AB  - In contrast to nodal follicular lymphoma, limited data exist on genetic changes 
      in primary cutaneous follicular lymphoma (primary cutaneous follicle center 
      lymphoma according to WHO-EORTC). The detection rate of the BCL2 rearrangement, 
      representing the characteristic t(14;18)(q32;q21) underlying follicular lymphoma, 
      by polymerase chain reaction (PCR) has been reported to vary over a wide range 
      (0%-41%), and only a few cases have been studied by molecular cytogenetic 
      techniques such as fluorescence in situ hybridization (FISH). In this study, 27 
      primary cutaneous follicle center lymphomas were analyzed by FISH and the results 
      compared with those obtained by PCR. FISH demonstrated translocations affecting 
      the immunoglobulin heavy chain locus (IGH) in 14 of 27 cases (52%): a 
      t(14;18)(q32;q21) involving BCL2 was found in 11 cases (41%), a t(3;14)(q27;q32) 
      affecting BCL6 in 2 cases (7%), and in 1 case the partner gene of IGH could not 
      be identified. Interestingly, PCR did not detect BCL2 rearrangement in any case. 
      These data suggest that the t(14;18)(q32;q21) frequently occurs in primary 
      cutaneous follicular lymphoma. The reason(s) why BCL2 rearrangements escape the 
      detection by PCR is (are) not clear but could be due to BCL2 mutations, 
      breakpoints outside the amplified DNA, or a high load of somatic mutations.
FAU - Streubel, Berthold
AU  - Streubel B
AD  - Department of Pathology, Medical University of Vienna, Vienna General Hospital, 
      Vienna, Austria.
FAU - Scheucher, Brigitte
AU  - Scheucher B
FAU - Valencak, Julia
AU  - Valencak J
FAU - Huber, Daniela
AU  - Huber D
FAU - Petzelbauer, Peter
AU  - Petzelbauer P
FAU - Trautinger, Franz
AU  - Trautinger F
FAU - Weihsengruber, Felix
AU  - Weihsengruber F
FAU - Mannhalter, Christine
AU  - Mannhalter C
FAU - Cerroni, Lorenzo
AU  - Cerroni L
FAU - Chott, Andreas
AU  - Chott A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Banding
MH  - *Chromosomes, Human, Pair 14
MH  - *Chromosomes, Human, Pair 18
MH  - Clone Cells
MH  - DNA, Neoplasm/analysis
MH  - Disease-Free Survival
MH  - Female
MH  - *Gene Rearrangement, B-Lymphocyte, Heavy Chain
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Follicular/genetics/*pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics
MH  - Proto-Oncogene Proteins c-bcl-6/genetics
MH  - Skin Neoplasms/genetics/*pathology/surgery
MH  - Translocation, Genetic
EDAT- 2006/04/21 09:00
MHDA- 2006/05/19 09:00
CRDT- 2006/04/21 09:00
PHST- 2006/04/21 09:00 [pubmed]
PHST- 2006/05/19 09:00 [medline]
PHST- 2006/04/21 09:00 [entrez]
AID - 00000478-200604000-00015 [pii]
AID - 10.1097/00000478-200604000-00015 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2006 Apr;30(4):529-36. doi: 10.1097/00000478-200604000-00015.