PMID- 1662554
OWN - NLM
STAT- MEDLINE
DCOM- 19920214
LR  - 20191028
IS  - 0169-6009 (Print)
IS  - 0169-6009 (Linking)
VI  - 15
IP  - 2
DP  - 1991 Nov
TI  - Epidermal growth factor attenuates cell proliferation by down-regulating the 
      transforming growth factor-beta receptor in the osteoblastic cell line MC3T3-E1.
PG  - 125-35
AB  - We investigated the role of transforming growth factor beta 1 (TGF-beta 1) in 
      growth regulation of the murine osteoblastic cell line, MC3T3-E1. We detected 
      TGF-beta activity in the conditioned medium of MC3T3-E1 cells and its activity 
      was increased by acid treatment of the bioassay system using CC1 64 cells. 
      Northern blot analysis revealed the expression of TGF-beta 1 precursor messenger 
      ribonucleic acid (mRNA). MC3T3-E1 cells possessed a single class of high affinity 
      TGF-beta 1 receptor (2.8 x 10(4) sites per cell, Kd = 196 pM). Cross-linking 
      studies revealed three specific TGF-beta receptors with molecular masses of 65, 
      95 and 280 kDa. Both TGF-beta and epidermal growth factor (EGF) stimulated 
      [3H]-thymidine incorporation into cells. EGF decreased the number of TGF-beta 
      receptor dose-dependently, and pretreatment of cells with 10 nM EGF attenuated 
      cell growth by TGF-beta. All these data suggested that TGF-beta might be an 
      autocrine growth factor in murine osteoblastic MC3T3-E1 cells and that EGF might 
      modulate the growth of cells by down-regulating the TGF-beta receptor level.
FAU - Jikihara, H
AU  - Jikihara H
AD  - Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
FAU - Ikegami, H
AU  - Ikegami H
FAU - Sakata, M
AU  - Sakata M
FAU - Morishige, K
AU  - Morishige K
FAU - Fujita, Y
AU  - Fujita Y
FAU - Kurachi, H
AU  - Kurachi H
FAU - Miyake, A
AU  - Miyake A
FAU - Tanizawa, O
AU  - Tanizawa O
FAU - Terakawa, N
AU  - Terakawa N
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Bone Miner
JT  - Bone and mineral
JID - 8610542
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 10028-17-8 (Tritium)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9007-49-2 (DNA)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - DNA/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation/*drug effects/genetics
MH  - Epidermal Growth Factor/*pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoblasts/*cytology/metabolism/ultrastructure
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Cell Surface/drug effects/*genetics
MH  - Receptors, Transforming Growth Factor beta
MH  - Thymidine/metabolism
MH  - Tritium
EDAT- 1991/11/01 00:00
MHDA- 1991/11/01 00:01
CRDT- 1991/11/01 00:00
PHST- 1991/11/01 00:00 [pubmed]
PHST- 1991/11/01 00:01 [medline]
PHST- 1991/11/01 00:00 [entrez]
AID - 10.1016/0169-6009(91)90003-i [doi]
PST - ppublish
SO  - Bone Miner. 1991 Nov;15(2):125-35. doi: 10.1016/0169-6009(91)90003-i.