PMID- 16625674
OWN - NLM
STAT- MEDLINE
DCOM- 20070712
LR  - 20060509
IS  - 1439-4235 (Print)
IS  - 1439-4235 (Linking)
VI  - 7
IP  - 5
DP  - 2006 May 12
TI  - Quantum-dot-labeled DNA probes for fluorescence in situ hybridization (FISH) in 
      the microorganism Escherichia coli.
PG  - 1062-7
AB  - Semiconductor quantum dots (QDs) as a kind of nonisotopic biological labeling 
      material have many unique fluorescent properties relative to conventional organic 
      dyes and fluorescent proteins, such as composition- and size-dependent absorption 
      and emission, a broad absorption spectrum, photostability, and single-dot 
      sensitivity. These properties make them a promising stable and sensitive label, 
      which can be used for long-term fluorescent tracking and subcellular location of 
      genes and proteins. Here, a simple approach for the construction of QD-labeled 
      DNA probes was developed by attaching thiol-ssDNA to QDs via a metal-thiol bond. 
      The as-prepared QD-labeled DNA probes had high dispersivity, bioactivity, and 
      specificity for hybridization. Based on such a kind of probe with a sequence 
      complementary to multiple clone sites in plasmid pUC18, fluorescence in situ 
      hybridization of the tiny bacterium Escherichia coli has been realized for the 
      first time.
FAU - Wu, Sheng-Mei
AU  - Wu SM
AD  - College of Chemistry and Molecular Sciences and State Key Laboratory of Virology, 
      Wuhan University, Wuhan 430072, P. R. China.
FAU - Zhao, Xiang
AU  - Zhao X
FAU - Zhang, Zhi-Ling
AU  - Zhang ZL
FAU - Xie, Hai-Yan
AU  - Xie HY
FAU - Tian, Zhi-Quan
AU  - Tian ZQ
FAU - Peng, Jun
AU  - Peng J
FAU - Lu, Zhe-Xue
AU  - Lu ZX
FAU - Pang, Dai-Wen
AU  - Pang DW
FAU - Xie, Zhi-Xiong
AU  - Xie ZX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Chemphyschem
JT  - Chemphyschem : a European journal of chemical physics and physical chemistry
JID - 100954211
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - DNA Probes/*chemistry
MH  - DNA, Single-Stranded/chemistry
MH  - Electrophoresis, Agar Gel
MH  - Escherichia coli/*genetics/metabolism
MH  - Fluorescent Dyes/chemistry
MH  - In Situ Hybridization, Fluorescence/*instrumentation/methods
MH  - Microscopy, Fluorescence
MH  - Models, Chemical
MH  - Nanotechnology/*instrumentation/*methods
MH  - Nucleic Acid Hybridization
MH  - Plasmids/metabolism
MH  - *Quantum Dots
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 2006/04/21 09:00
MHDA- 2007/07/13 09:00
CRDT- 2006/04/21 09:00
PHST- 2006/04/21 09:00 [pubmed]
PHST- 2007/07/13 09:00 [medline]
PHST- 2006/04/21 09:00 [entrez]
AID - 10.1002/cphc.200500608 [doi]
PST - ppublish
SO  - Chemphyschem. 2006 May 12;7(5):1062-7. doi: 10.1002/cphc.200500608.