PMID- 16626638
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20061115
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1086
IP  - 1
DP  - 2006 May 1
TI  - Chronic stress induces upregulation of brain-derived neurotrophic factor (BDNF) 
      mRNA and integrin alpha5 expression in the rat pineal gland.
PG  - 27-34
AB  - Chronic stress affects brain areas involved in learning and emotional responses. 
      These alterations have been related with the development of cognitive deficits in 
      major depression. Moreover, stress induces deleterious actions on the epithalamic 
      pineal organ, a gland involved in a wide range of physiological functions. The 
      aim of this study was to investigate whether the stress effects on the pineal 
      gland are related with changes in the expression of neurotrophic factors and cell 
      adhesion molecules. Using reverse transcription-polymerase chain reaction 
      (RT-PCR) and Western blot, we analyzed the effect of chronic immobilization 
      stress on the BDNF mRNA and integrin alpha5 expression in the rat pineal gland. 
      We found that BDNF is produced in situ in the pineal gland. Chronic 
      immobilization stress induced upregulation of BDNF mRNA and integrin alpha5 
      expression in the rat pineal gland but did not produce changes in beta-actin mRNA 
      or in GAPDH expression. Stressed animals also evidenced an increase in 
      anxiety-like behavior and acute gastric lesions. These results suggest that BDNF 
      and integrin alpha5 may have a counteracting effect to the deleterious actions of 
      immobilization stress on functionally stimulated pinealocytes. Furthermore, this 
      study proposes that the pineal gland may be a target of glucocorticoid damage 
      during stress.
FAU - Dagnino-Subiabre, Alexies
AU  - Dagnino-Subiabre A
AD  - Department of Psychiatry and Center for Medical Research, Faculty of Medicine, 
      Pontificia Universidad Catolica de Chile, Ave. Marcoleta N 387, piso 2, Casilla 
      114-D, Santiago 1, Chile. adagnino@med.puc.cl
FAU - Zepeda-Carreno, Rodrigo
AU  - Zepeda-Carreno R
FAU - Diaz-Veliz, Gabriela
AU  - Diaz-Veliz G
FAU - Mora, Sergio
AU  - Mora S
FAU - Aboitiz, Francisco
AU  - Aboitiz F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060413
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Integrin alpha5)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Blotting, Western/methods
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Gene Expression/*physiology
MH  - Integrin alpha5/genetics/*metabolism
MH  - Male
MH  - Maze Learning/physiology
MH  - Motor Activity/physiology
MH  - Pineal Gland/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Restraint, Physical/methods
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Stress, Psychological/*physiopathology
MH  - Up-Regulation/*physiology
EDAT- 2006/04/22 09:00
MHDA- 2006/08/11 09:00
CRDT- 2006/04/22 09:00
PHST- 2005/05/05 00:00 [received]
PHST- 2006/01/25 00:00 [revised]
PHST- 2006/02/26 00:00 [accepted]
PHST- 2006/04/22 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2006/04/22 09:00 [entrez]
AID - S0006-8993(06)00601-9 [pii]
AID - 10.1016/j.brainres.2006.02.118 [doi]
PST - ppublish
SO  - Brain Res. 2006 May 1;1086(1):27-34. doi: 10.1016/j.brainres.2006.02.118. Epub 
      2006 Apr 13.