PMID- 16626872
OWN - NLM
STAT- MEDLINE
DCOM- 20060908
LR  - 20220310
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 140
IP  - 3
DP  - 2006 Jul 7
TI  - Patterns of expression of brain-derived neurotrophic factor and tyrosine kinase B 
      mRNAs and distribution and ultrastructural localization of their proteins in the 
      visual pathway of the adult rat.
PG  - 913-28
AB  - We have examined the cellular and subcellular distribution and the patterns of 
      expression of brain-derived neurotrophic factor (BDNF), and of its high affinity 
      receptor, tyrosine kinase B (TrkB), in retinorecipient regions of the brain, 
      including the superior colliculus, the lateral geniculate nucleus and the olivary 
      pretectal nucleus. In the retinorecipient layers of the superior colliculus, BDNF 
      protein and mRNA were present in the cell bodies of a subpopulation of neurons, 
      and BDNF protein was present in the neuropil as punctate or fiber-like 
      structures. In the lateral geniculate nucleus, however, BDNF mRNA was not 
      detected, and BDNF protein was restricted to punctate and fiber-like structures 
      in the neuropil, especially in the most superficial part of the dorsal lateral 
      geniculate nucleus, just below the optic tract. At the ultrastructural level, 
      BDNF protein was localized predominantly to axon terminals containing round 
      synaptic vesicles and pale mitochondria with irregular cristae, which made 
      asymmetric (Gray type I) synaptic specializations (R-boutons). Enucleation of one 
      eye was followed by loss of BDNF immunoreactivity and disappearance of 
      BDNF-positive R-boutons in the contralateral visual centers, confirming the 
      retinal origin of at least most of these terminals. TrkB was present in 
      postsynaptic densities apposed to immunoreactive R-boutons in the superior 
      colliculus and lateral geniculate nucleus, and was also associated with axonal 
      and dendritic microtubules. These findings suggest that BDNF is synthesized by a 
      subpopulation of retinal ganglion cells and axonally transported to visual 
      centers where this neurotrophin is assumed to play important roles in visual 
      system maintenance and/or in modulating the excitatory retinal input to neurons 
      in these centers.
FAU - Avwenagha, O
AU  - Avwenagha O
AD  - Department of Anatomy and Developmental Biology, University College London, Gower 
      Street, London WC1E 6BT, UK.
FAU - Bird, M M
AU  - Bird MM
FAU - Lieberman, A R
AU  - Lieberman AR
FAU - Yan, Q
AU  - Yan Q
FAU - Campbell, G
AU  - Campbell G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060419
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Axonal Transport/physiology
MH  - Brain/*metabolism/ultrastructure
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Dendrites/metabolism/ultrastructure
MH  - Female
MH  - Geniculate Bodies/metabolism/ultrastructure
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Microtubules/metabolism/ultrastructure
MH  - Mitochondria/metabolism/ultrastructure
MH  - Neuropil/metabolism/ultrastructure
MH  - Presynaptic Terminals/*metabolism/ultrastructure
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/*genetics
MH  - Retinal Ganglion Cells/metabolism/ultrastructure
MH  - Superior Colliculi/metabolism/ultrastructure
MH  - Synaptic Membranes/metabolism/ultrastructure
MH  - Synaptic Vesicles/metabolism/ultrastructure
MH  - Visual Pathways/*metabolism/ultrastructure
EDAT- 2006/04/22 09:00
MHDA- 2006/09/09 09:00
CRDT- 2006/04/22 09:00
PHST- 2005/05/25 00:00 [received]
PHST- 2006/02/23 00:00 [revised]
PHST- 2006/02/23 00:00 [accepted]
PHST- 2006/04/22 09:00 [pubmed]
PHST- 2006/09/09 09:00 [medline]
PHST- 2006/04/22 09:00 [entrez]
AID - S0306-4522(06)00296-X [pii]
AID - 10.1016/j.neuroscience.2006.02.056 [doi]
PST - ppublish
SO  - Neuroscience. 2006 Jul 7;140(3):913-28. doi: 10.1016/j.neuroscience.2006.02.056. 
      Epub 2006 Apr 19.