PMID- 16628748
OWN - NLM
STAT- MEDLINE
DCOM- 20060718
LR  - 20061115
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 47
IP  - 5
DP  - 2006 Jun
TI  - In vivo micronuclei in uncultured T-lymphocytes of male railroad transit workers 
      and referents.
PG  - 345-51
AB  - In the biomonitoring of human genotoxic effects, micronuclei (MN) usually are 
      scored in phytohaemagglutinin-stimulated cultured lymphocytes. MN also can be 
      examined in uncultured lymphocytes, which facilitates the analysis of genotoxic 
      damage incurred in vivo. Characterization of MN in cultured lymphocytes by 
      fluorescence in situ hybridization (FISH) has shown a clear over-representation 
      of the X and Y chromosomes in the MN of males. However, it is not known if this 
      phenomenon also occurs in vivo. The purpose of the present study was to assess 
      the frequency and composition of MN formed in vivo from immunomagnetically 
      isolated uncultured T-lymphocytes of men. To evaluate the possible effects of 
      genotoxic exposure on in vivo MN, we examined 17 railroad workers occupationally 
      exposed to complex chemical mixtures and 14 referents, all nonsmokers. The 
      results showed similar total frequencies of micronucleated cells among the 
      exposed workers and the referents. When the MN were characterized by FISH, there 
      were no significant differences between the exposed and referents with regards to 
      the frequency of centromere-positive or centromere-negative MN. Centromeric label 
      was observed in 69% of all MN, indicating that most of the MN contained whole 
      chromosomes (or chromatids). 80% of the centromere-positive MN harbored 
      autosomes, 12% Y chromosomes, and 8% X chromosomes. The occurrence of the Y- and 
      X-chromosomes in MN was, respectively, 5.5- and 3.8-times greater than would be 
      expected assuming an equal contribution by all chromosomes. Thus, sex chromosomes 
      appear to be over-represented in lymphocyte MN of men in vivo, confirming 
      previous results obtained in vitro.
FAU - Catalan, Julia
AU  - Catalan J
AD  - Finnish Institute of Occupational Health, Helsinki and Lappeenranta, Finland.
FAU - Falck, Ghita C-M
AU  - Falck GC
FAU - Jarventaus, Hilkka
AU  - Jarventaus H
FAU - Kallas-Tarpila, Tarja
AU  - Kallas-Tarpila T
FAU - Pitkamaki, Leena
AU  - Pitkamaki L
FAU - Norppa, Hannu
AU  - Norppa H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (Hydrocarbons)
RN  - 0 (Petroleum)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Centromere
MH  - Chromosomes, Human, X
MH  - Chromosomes, Human, Y
MH  - Humans
MH  - Hydrocarbons
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Micronuclei, Chromosome-Defective
MH  - Micronucleus Tests/methods
MH  - Middle Aged
MH  - Occupational Exposure/*adverse effects
MH  - Petroleum
MH  - *Railroads
MH  - T-Lymphocytes/*drug effects
EDAT- 2006/04/22 09:00
MHDA- 2006/07/19 09:00
CRDT- 2006/04/22 09:00
PHST- 2006/04/22 09:00 [pubmed]
PHST- 2006/07/19 09:00 [medline]
PHST- 2006/04/22 09:00 [entrez]
AID - 10.1002/em.20213 [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 2006 Jun;47(5):345-51. doi: 10.1002/em.20213.