PMID- 16629755
OWN - NLM
STAT- MEDLINE
DCOM- 20060630
LR  - 20240109
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 56
IP  - 2
DP  - 2006 May
TI  - Colonization of barley (Hordeum vulgare) with Salmonella enterica and Listeria 
      spp.
PG  - 262-71
AB  - Colonization of barley plants by the food-borne pathogens Salmonella enterica 
      serovar typhimurium and three Listeria spp. (L. monocytogenes, L. ivanovii, L. 
      innocua) was investigated in a monoxenic system. Herbaspirillum sp. N3 was used 
      as a positive control and Escherichia coli HB101 as a negative control for 
      endophytic root colonization. Colonization of the plants was tested 1-4 weeks 
      after inoculation by determination of CFU, specific PCR assays and fluorescence 
      in situ hybridization (FISH) with fluorescently labelled oligonucleotide probes 
      in combination with confocal laser scanning microscopy (CLSM). Both S. enterica 
      strains were found as endophytic colonizers of barley roots and reached up to 2.3 
      x 10(6) CFU per g root fresh weight after surface sterilization. The three 
      Listeria strains had 10-fold fewer cell numbers after surface sterilization on 
      the roots and therefore were similar to the results of nonendophytic colonizers, 
      such as E. coli HB101. The FISH/CSLM approach demonstrated not only high-density 
      colonization of the root hairs and the root surface by S. enterica but also a 
      spreading to subjacent rhizodermis layers and the inner root cortex. By contrast, 
      the inoculated Listeria spp. colonized the root hair zone but did not colonize 
      other parts of the root surface. Endophytic colonization of Listeria spp. was not 
      observed. Finally, a systemic spreading of S. enterica to the plant shoot (stems 
      and leaves) was demonstrated using a specific PCR analysis and plate count 
      technique.
FAU - Kutter, Stefan
AU  - Kutter S
AD  - GSF-National Research Center for Environment and Health, Department of 
      Rhizosphere Biology, Institute of Soil Ecology, Neuherberg, Germany.
FAU - Hartmann, Anton
AU  - Hartmann A
FAU - Schmid, Michael
AU  - Schmid M
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
SB  - IM
MH  - Hordeum/cytology/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Listeria/cytology/growth & development/*isolation & purification
MH  - Plant Roots/cytology/microbiology
MH  - Plant Shoots/cytology/microbiology
MH  - Polymerase Chain Reaction
MH  - Salmonella enterica/cytology/growth & development/*isolation & purification
MH  - Seedlings/microbiology
EDAT- 2006/04/25 09:00
MHDA- 2006/07/01 09:00
CRDT- 2006/04/25 09:00
PHST- 2006/04/25 09:00 [pubmed]
PHST- 2006/07/01 09:00 [medline]
PHST- 2006/04/25 09:00 [entrez]
AID - FEM053 [pii]
AID - 10.1111/j.1574-6941.2005.00053.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2006 May;56(2):262-71. doi: 
      10.1111/j.1574-6941.2005.00053.x.