PMID- 16629789
OWN - NLM
STAT- MEDLINE
DCOM- 20061012
LR  - 20211020
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 61
IP  - 5
DP  - 2006 May
TI  - Human airway and peripheral blood eosinophils enhance Th1 and Th2 cytokine 
      secretion.
PG  - 589-97
AB  - BACKGROUND: The effector function of eosinophils involves their release of toxic 
      granule proteins, reactive oxygen species, cytokines, and lipid mediators. Murine 
      studies have demonstrated that eosinophils can also enhance T cell function. 
      Whether human eosinophils, in particular, airway eosinophils, have similar 
      immunoregulatory activity has not been fully investigated. The aim of this study 
      was to determine whether human blood and airway eosinophils can contribute to Th1 
      and Th2 cytokine generation from CD4+ T cells stimulated with superantigen. 
      METHODS: Eosinophils were obtained from blood or bronchoalveolar lavage fluid 48 
      h after segmental allergen bronchoprovocation. Purified eosinophils were 
      co-cultured with autologous CD4+ blood T cells in the presence of staphylococcal 
      enterotoxin B (SEB). Cytokine levels in the supernatant fluid were determined by 
      enzyme-linked immunosorbent assay (ELISA). Eosinophil expression of major 
      histocompatibility complex (MHC) class II and co-stimulatory molecules was 
      assessed by flow cytometry before culture, 24 h after granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) stimulation, and 24 h after co-culture with 
      CD4+ T cells and SEB. RESULTS: Interleukin (IL)-5, IL-13, and interferon 
      (IFN)-gamma generation increased when CD4+ T cells were co-cultured with either 
      blood or airway eosinophils in the presence of SEB. The ability of eosinophils to 
      enhance cytokine generation was independent of their source (blood vs airway), 
      activation by GM-CSF, or detectable expression of human leukocyte antigen 
      (HLA)-DR, CD80, or CD86. CONCLUSION: Our data demonstrate that SEB-induced 
      generation of Th1 and Th2 cytokines is increased in the presence of human blood 
      and airway eosinophils. Thus, eosinophils can have an immunoregulatory function 
      in pathogen-associated allergic diseases such as atopic dermatitis, chronic 
      sinusitis, and asthma exacerbations.
FAU - Liu, L-Y
AU  - Liu LY
AD  - Section of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, 
      University of Wisconsin, Madison, WI 53792, USA.
FAU - Mathur, S K
AU  - Mathur SK
FAU - Sedgwick, J B
AU  - Sedgwick JB
FAU - Jarjour, N N
AU  - Jarjour NN
FAU - Busse, W W
AU  - Busse WW
FAU - Kelly, E A B
AU  - Kelly EA
LA  - eng
GR  - P50 HL056396/HL/NHLBI NIH HHS/United States
GR  - M01 RR003186/RR/NCRR NIH HHS/United States
GR  - HL56936/HL/NHLBI NIH HHS/United States
GR  - L30 HL078001-02/HL/NHLBI NIH HHS/United States
GR  - M01 RR03186/RR/NCRR NIH HHS/United States
GR  - L30 HL078001/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Cytokines)
RN  - 0 (Enterotoxins)
RN  - 0 (Interleukin-5)
RN  - 39424-53-8 (enterotoxin B, staphylococcal)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Blood Cells/physiology
MH  - *Bronchoalveolar Lavage Fluid
MH  - CD4-Positive T-Lymphocytes/metabolism
MH  - Cells, Cultured
MH  - Cytokines/*metabolism
MH  - Enterotoxins/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eosinophils/*physiology
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Interferons/metabolism
MH  - Interleukin-5/metabolism
MH  - Th1 Cells/immunology/*metabolism
MH  - Th2 Cells/immunology/*metabolism
EDAT- 2006/04/25 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/04/25 09:00
PHST- 2006/04/25 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/04/25 09:00 [entrez]
AID - ALL1060 [pii]
AID - 10.1111/j.1398-9995.2006.01060.x [doi]
PST - ppublish
SO  - Allergy. 2006 May;61(5):589-97. doi: 10.1111/j.1398-9995.2006.01060.x.