PMID- 16631119
OWN - NLM
STAT- MEDLINE
DCOM- 20060622
LR  - 20121115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 344
IP  - 3
DP  - 2006 Jun 9
TI  - TNFR-Fc fusion protein expressed by in vivo electroporation improves survival 
      rates and myocardial injury in coxsackievirus induced murine myocarditis.
PG  - 765-71
AB  - Tumor necrosis factor-alpha (TNF-alpha) is one of the major cytokines that 
      modulate the immune response in viral myocarditis, but its role has not yet been 
      thoroughly evaluated. We antagonized TNF-alpha using the expressed soluble p75 
      TNF receptor linked to the Fc portion of the human IgG1 gene (sTNFR:Fc) by in 
      vivo electroporation, and evaluated its effects on experimental coxsackieviral B3 
      (CVB3) myocarditis. A plasmid DNA encoding sTNFR:Fc (15microg/mouse) was injected 
      into the gastrocnemius muscles of Balb/C male mice followed by electroporation 
      (day -1). Control mice were injected with an empty vector. One day after 
      electroporation, mice were infected with CVB3 (day 0). Serum levels of sTNFR:Fc 
      increased from day 2 and peaked at day 5 following electroporation. The heart 
      virus titers of sTNFR:Fc mice were higher than those of controls at day 3. 
      However, subsequent to day 12, the survival rates of the sTNFR:Fc mice were 
      significantly higher than those of the controls (36% versus 0% at day 27, 
      P<0.01). Histopathological examination indicated that inflammation and myocardial 
      fibrosis were significantly decreased in sTNFR:Fc mice at day 12. The expressed 
      sTNFR:Fc could modulate the inflammatory process during the post-viremic phase of 
      viral myocarditis.
FAU - Kim, Jong-Mook
AU  - Kim JM
AD  - ViroMed Co. Ltd, Seoul, Republic of Korea.
FAU - Lim, Byung-Kwan
AU  - Lim BK
FAU - Ho, Seong-Hyun
AU  - Ho SH
FAU - Yun, Soo-Hyeon
AU  - Yun SH
FAU - Shin, Jae-Ok
AU  - Shin JO
FAU - Park, Eun-Min
AU  - Park EM
FAU - Kim, Duk-Kyung
AU  - Kim DK
FAU - Kim, Sunyoung
AU  - Kim S
FAU - Jeon, Eun-Seok
AU  - Jeon ES
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060405
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Animals
MH  - Coxsackievirus Infections/metabolism/*pathology/*therapy
MH  - Electroporation/methods
MH  - Genetic Therapy/methods
MH  - Immunoglobulin G/*administration & dosage/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Myocarditis/metabolism/*pathology/*therapy/virology
MH  - Receptors, Tumor Necrosis Factor/*administration & dosage/*genetics
MH  - Recombinant Fusion Proteins/administration & dosage
MH  - Survival Analysis
MH  - Survival Rate
MH  - Transfection/methods
MH  - Treatment Outcome
EDAT- 2006/04/25 09:00
MHDA- 2006/06/23 09:00
CRDT- 2006/04/25 09:00
PHST- 2006/03/13 00:00 [received]
PHST- 2006/03/22 00:00 [accepted]
PHST- 2006/04/25 09:00 [pubmed]
PHST- 2006/06/23 09:00 [medline]
PHST- 2006/04/25 09:00 [entrez]
AID - S0006-291X(06)00679-6 [pii]
AID - 10.1016/j.bbrc.2006.03.170 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Jun 9;344(3):765-71. doi: 
      10.1016/j.bbrc.2006.03.170. Epub 2006 Apr 5.