PMID- 16631472
OWN - NLM
STAT- MEDLINE
DCOM- 20060525
LR  - 20071115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 166
IP  - 2
DP  - 2006 Apr 15
TI  - Metaphase cells with normal G-bands have cryptic interstitial deletions in 13q14 
      detectable by fluorescence in situ hybridization in B-cell chronic lymphocytic 
      leukemia.
PG  - 152-6
AB  - Interphase fluorescence in situ hybridization (FISH) studies with D13S319 show 
      that deletions of 13q14 are common in B-cell chronic lymphocytic leukemia 
      (B-CLL). In contrast, conventional cytogenetic studies in B-CLL seldom reveal 
      abnormalities of chromosome 13. We hypothesized that chromosome 13 anomalies 
      might not be detected because they are caused by cryptic deletions rather than by 
      the absence of dividing B-CLL cells. To investigate this possibility, we used 
      FISH with D13S319 to study metaphases from 12 patients known to have 13q- by 
      interphase FISH. These same patients had normal chromosomes by conventional 
      cytogenetic studies. As a result of this study, we report evidence that B-CLL 
      metaphases with 13q- are not detected because these deletions are often cryptic 
      and not visible by standard G-banding.
FAU - Stockero, Kimberly J
AU  - Stockero KJ
AD  - Cytogenetics Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
FAU - Fink, Stephanie R
AU  - Fink SR
FAU - Smoley, Stephanie A
AU  - Smoley SA
FAU - Paternoster, Sarah F
AU  - Paternoster SF
FAU - Shanafelt, Tait D
AU  - Shanafelt TD
FAU - Call, Timothy G
AU  - Call TG
FAU - Zent, Clive S
AU  - Zent CS
FAU - Van Dyke, Daniel L
AU  - Van Dyke DL
FAU - Kay, Neil E
AU  - Kay NE
FAU - Dewald, Gordon W
AU  - Dewald GW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Chromosome Banding/*methods
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 13/*genetics
MH  - Cohort Studies
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/*pathology
MH  - Male
MH  - Metaphase/*genetics
MH  - Middle Aged
EDAT- 2006/04/25 09:00
MHDA- 2006/05/26 09:00
CRDT- 2006/04/25 09:00
PHST- 2005/09/01 00:00 [received]
PHST- 2005/10/25 00:00 [revised]
PHST- 2005/10/31 00:00 [accepted]
PHST- 2006/04/25 09:00 [pubmed]
PHST- 2006/05/26 09:00 [medline]
PHST- 2006/04/25 09:00 [entrez]
AID - S0165-4608(05)00663-1 [pii]
AID - 10.1016/j.cancergencyto.2005.10.011 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2006 Apr 15;166(2):152-6. doi: 
      10.1016/j.cancergencyto.2005.10.011.