PMID- 16631618 OWN - NLM STAT- MEDLINE DCOM- 20060622 LR - 20061115 IS - 0006-291X (Print) IS - 0006-291X (Linking) VI - 344 IP - 3 DP - 2006 Jun 9 TI - Autocrine activation of cultured macrophages by brain-derived neurotrophic factor. PG - 941-7 AB - To elucidate a significance of the expression of brain-derived neurotrophic factor (BDNF) in the activated microglia/macrophages of the injured central nervous system, we examined BDNF actions on or BDNF synthesis by macrophages cultured from the mouse peritoneal cavity. They synthesized BDNF and neurotrophin-3 (NT-3) in addition to expressing high-affinity neurotrophin receptors, full-length TrkB (FL), truncated TrkB (TK(-)), and TrkC, thus suggesting an autocrine influence of BDNF and NT-3. BDNF, but not NT-3, enhanced phagocytic activity and stimulated synthesis/secretion of interleukin-1beta in the same manner as lipopolysaccharide (LPS). Furthermore, there was a significant correlation of the phagocytic activity with the expression of BDNF or TrkB (FL). These results imply that the phagocytic activity of macrophages depends on BDNF synthesis and/or TrkB (FL) expression, suggesting that BDNF participates in the activation processes of macrophages by acting in an autocrine manner. FAU - Asami, Toshio AU - Asami T AD - Laboratory of Molecular Biology, Gifu Pharmaceutical University, 5-6-1, Mitahora-Higashi, Gifu 502-8585, Japan. FAU - Ito, Takuya AU - Ito T FAU - Fukumitsu, Hidefumi AU - Fukumitsu H FAU - Nomoto, Hiroshi AU - Nomoto H FAU - Furukawa, Yoshiko AU - Furukawa Y FAU - Furukawa, Shoei AU - Furukawa S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20060419 PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Interleukin-1) RN - 0 (Neurotrophin 3) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*administration & dosage/*metabolism MH - Cells, Cultured MH - Dose-Response Relationship, Drug MH - Interleukin-1/*metabolism MH - Macrophage Activation MH - Macrophages/drug effects/*physiology MH - Mice MH - Neurotrophin 3/*administration & dosage MH - Phagocytosis/drug effects/*physiology EDAT- 2006/04/25 09:00 MHDA- 2006/06/23 09:00 CRDT- 2006/04/25 09:00 PHST- 2006/03/16 00:00 [received] PHST- 2006/03/30 00:00 [accepted] PHST- 2006/04/25 09:00 [pubmed] PHST- 2006/06/23 09:00 [medline] PHST- 2006/04/25 09:00 [entrez] AID - S0006-291X(06)00765-0 [pii] AID - 10.1016/j.bbrc.2006.03.228 [doi] PST - ppublish SO - Biochem Biophys Res Commun. 2006 Jun 9;344(3):941-7. doi: 10.1016/j.bbrc.2006.03.228. Epub 2006 Apr 19.