PMID- 16635388
OWN - NLM
STAT- MEDLINE
DCOM- 20070807
LR  - 20131121
IS  - 0529-5815 (Print)
IS  - 0529-5815 (Linking)
VI  - 44
IP  - 5
DP  - 2006 Mar 1
TI  - [The protective effect of heat shock protein 72 by Doxorubicin in cold 
      ischemia-reperfusion injury of the rat liver].
PG  - 310-3
AB  - OBJECTIVE: To observe induction of heat shock reaction by pretreatment of 
      Doxorubicin (DXR) in long-term cold preservation-reperfusion injury of the rat 
      liver. METHODS: The rats were administered intravenously by DXR at a dose of 1 
      mg/kg body weight in DXR group and by saline in control group. After 48 hours, 
      the rat liver was perfused by using cold University of Wisconsin (UW) solutions 
      and was preserved in UW solution at 4 degrees C for 48 hours. Recipient liver was 
      perfused for 1 and 3 hours after orthotopic liver transplantation. Tumor necrosis 
      factor-alpha (TNF-alpha) mRNA, cytokine-induced neutrophil chemoattractant (CINC) 
      mRNA, macrophage inflammatory protein (MIP-2) mRNA was measured by RT-PCR and 
      heat shock protein 72 (HSP72), nuclear factor-kappaB (NF-kappaB) by Western blot. 
      The serum levels of TNF-alpha, CINC, MIP-2 by ELISA and AST were measured. The 
      survival rate of 7 days was observed. RESULTS: The expression of TNF-alpha mRNA, 
      CINC mRNA and MIP-2 mRNA was stronger in control group than in DXR group. HSP72 
      was expressed in SA group but not in control group and oppositely NF-kappaB was 
      expressed in control group but not in DXR group. Serum AST, TNF-alpha, CINC and 
      MIP-2 concentrations were significantly lower in DXR group than in control group 
      (P < 0.05). The survival rate of 7 days was significantly higher in DXR group 
      than in control group (50% vs. 0%, P < 0.05). CONCLUSIONS: These data suggested 
      that long-term cold ischemia-reperfusion injury was attenuated in liver graft 
      with pretreatment of DXR. The induction of HSP72 may offer protection to 
      hepatocytes by restraining the activation of NF-kappaB and inflammation.
FAU - Chen, Hao
AU  - Chen H
AD  - Center of Organ Transplantation, Ruijin Hospital, Medical School of Shanghai 
      Jiaotong University, Shanghai 200025, China.
FAU - Peng, Cheng-hong
AU  - Peng CH
FAU - Deng, Xia-xing
AU  - Deng XX
FAU - Qiu, Wei-hua
AU  - Qiu WH
FAU - Shen, Bai-yong
AU  - Shen BY
FAU - Yang, Wei-ping
AU  - Yang WP
FAU - Li, Hong-wei
AU  - Li HW
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wai Ke Za Zhi
JT  - Zhonghua wai ke za zhi [Chinese journal of surgery]
JID - 0153611
RN  - 0 (Chemokines, CXC)
RN  - 0 (HSP72 Heat-Shock Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Animals
MH  - Chemokines, CXC/biosynthesis/genetics
MH  - Cryopreservation
MH  - Doxorubicin/*pharmacology
MH  - HSP72 Heat-Shock Proteins/*biosynthesis
MH  - Liver/*blood supply/drug effects/metabolism
MH  - Liver Transplantation
MH  - Male
MH  - RNA, Messenger/genetics
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/etiology/*prevention & control
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Survival
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics
EDAT- 2006/04/26 09:00
MHDA- 2007/08/08 09:00
CRDT- 2006/04/26 09:00
PHST- 2006/04/26 09:00 [pubmed]
PHST- 2007/08/08 09:00 [medline]
PHST- 2006/04/26 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Wai Ke Za Zhi. 2006 Mar 1;44(5):310-3.