PMID- 16635492
OWN - NLM
STAT- MEDLINE
DCOM- 20060824
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1118
IP  - 1
DP  - 2006 Jun 16
TI  - Selenium speciation by high-performance anion-exchange chromatography-post-column 
      UV irradiation coupled with atomic fluorescence spectrometry.
PG  - 139-43
AB  - A technique for the speciation of selenomethylcysteine (SeMeCys), selenocystine 
      (SeCys), selenite [Se(IV)] and selenomethionine (SeMet) was established in this 
      paper using high-performance anion-exchange chromatography coupled with atomic 
      fluorescence spectrometry (HPAEC-AFS). Analytes were separated on an AminoPac 
      PA10 column and then digested by on-line ultraviolet (UV) irradiation, which 
      destroyed organic compound structure. Hydride generation was used as an available 
      sample introduction technique for atomic fluorescence detection. The detection 
      limits of four compounds were 1-5 microg/L (250 microL injection, 10 times of the 
      baseline noise). The relative standard deviations (RSDs), calculated from seven 
      consecutive injections of 100 microg/L standard mixtures, were from 2 to 4%. 
      Selenious yeast tablet, which had been proposed as selenium supplement, and human 
      urine collected from a volunteer were analyzed. Good spiked recoveries from 86 to 
      103% were obtained.
FAU - Liang, Lina
AU  - Liang L
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 
      2871, Beijing 100085, China.
FAU - Mo, Shumin
AU  - Mo S
FAU - Zhang, Ping
AU  - Zhang P
FAU - Cai, Yaqi
AU  - Cai Y
FAU - Mou, Shifen
AU  - Mou S
FAU - Jiang, Guibin
AU  - Jiang G
FAU - Wen, Meijuan
AU  - Wen M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060425
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Ion Exchange Resins)
RN  - 0 (Organoselenium Compounds)
RN  - 0 (Tablets)
RN  - 1464-43-3 (selenocystine)
RN  - 48TCX9A1VT (Cystine)
RN  - 55X04QC32I (Sodium Hydroxide)
RN  - 964MRK2PEL (Selenomethionine)
RN  - H6241UJ22B (Selenium)
RN  - HIW548RQ3W (Sodium Selenite)
RN  - QTT17582CB (Hydrochloric Acid)
SB  - IM
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Cystine/analogs & derivatives/analysis
MH  - Humans
MH  - Hydrochloric Acid/chemistry
MH  - Ion Exchange Resins/chemistry
MH  - Organoselenium Compounds/analysis/urine
MH  - Reproducibility of Results
MH  - Selenium/*analysis/urine
MH  - Selenomethionine/analysis/urine
MH  - Sodium Hydroxide/chemistry
MH  - Sodium Selenite/analysis/urine
MH  - Spectrometry, Fluorescence/instrumentation/*methods
MH  - Spectrophotometry, Atomic/instrumentation/*methods
MH  - Tablets/chemistry
MH  - Ultraviolet Rays
MH  - Yeasts/chemistry
EDAT- 2006/04/26 09:00
MHDA- 2006/08/25 09:00
CRDT- 2006/04/26 09:00
PHST- 2005/10/26 00:00 [received]
PHST- 2006/03/29 00:00 [revised]
PHST- 2006/03/29 00:00 [accepted]
PHST- 2006/04/26 09:00 [pubmed]
PHST- 2006/08/25 09:00 [medline]
PHST- 2006/04/26 09:00 [entrez]
AID - S0021-9673(06)00737-0 [pii]
AID - 10.1016/j.chroma.2006.03.113 [doi]
PST - ppublish
SO  - J Chromatogr A. 2006 Jun 16;1118(1):139-43. doi: 10.1016/j.chroma.2006.03.113. 
      Epub 2006 Apr 25.