PMID- 16636109
OWN - NLM
STAT- MEDLINE
DCOM- 20060719
LR  - 20120215
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 20
IP  - 8
DP  - 2006 Jun
TI  - Delayed GM-CSF treatment stimulates axonal regeneration and functional recovery 
      in paraplegic rats via an increased BDNF expression by endogenous macrophages.
PG  - 1239-41
AB  - Macrophages (monocytes/microglia) could play a critical role in central nervous 
      system repair. We have previously found a synchronism between the regression of 
      spontaneous axonal regeneration and the deactivation of macrophages 3-4 wk after 
      a compression-injury of rat spinal cord. To explore whether reactivation of 
      endogenous macrophages might be beneficial for spinal cord repair, we have 
      studied the effects of granulocyte-macrophage colony stimulating factor (GM-CSF) 
      in the same paraplegia model and in cell cultures. There was a significant, 
      though transient, improvement of locomotor recovery after a single delayed 
      intraperitoneal injection of 2 microg GM-CSF, which also increased significantly 
      the expression of Cr3 and brain-derived neurotrophic factor (BDNF) by macrophages 
      at the lesion site. At longer survival delays, axonal regeneration was 
      significantly enhanced in GM-CSF-treated rats. In vitro, BV2 microglial cells 
      expressed higher levels of BDNF in the presence of GM-CSF and neurons cocultured 
      with microglial cells activated by GM-CSF generated more neurites, an effect 
      blocked by a BDNF antibody. These experiments suggest that GM-CSF could be an 
      interesting treatment option for spinal cord injury and that its beneficial 
      effects might be mediated by BDNF.
FAU - Bouhy, Delphine
AU  - Bouhy D
AD  - Research Center for Cellular and Molecular Neurobiology, University of Liege, 
      Tour de Pathologie B36, 1etage, local 1/4A, CHU Sart-Tilman 4000 Liege, Belgium.
FAU - Malgrange, Brigitte
AU  - Malgrange B
FAU - Multon, Sylvie
AU  - Multon S
FAU - Poirrier, Anne-Lise
AU  - Poirrier AL
FAU - Scholtes, Felix
AU  - Scholtes F
FAU - Schoenen, Jean
AU  - Schoenen J
FAU - Franzen, Rachelle
AU  - Franzen R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060424
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Axons/*drug effects/physiology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*therapeutic use
MH  - Kinetics
MH  - Macrophages/metabolism
MH  - Microglia/*metabolism
MH  - Motor Activity/drug effects
MH  - Nerve Fibers/drug effects/pathology/physiology
MH  - Nerve Regeneration/*drug effects
MH  - Neurites/drug effects
MH  - Paraplegia/*drug therapy/metabolism/physiopathology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2006/04/26 09:00
MHDA- 2006/07/20 09:00
CRDT- 2006/04/26 09:00
PHST- 2006/04/26 09:00 [pubmed]
PHST- 2006/07/20 09:00 [medline]
PHST- 2006/04/26 09:00 [entrez]
AID - fj.05-4382fje [pii]
AID - 10.1096/fj.05-4382fje [doi]
PST - ppublish
SO  - FASEB J. 2006 Jun;20(8):1239-41. doi: 10.1096/fj.05-4382fje. Epub 2006 Apr 24.