PMID- 16636668
OWN - NLM
STAT- MEDLINE
DCOM- 20061018
LR  - 20220318
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 25
IP  - 41
DP  - 2006 Sep 14
TI  - High-resolution analysis of chromosome rearrangements on 8p in breast, colon and 
      pancreatic cancer reveals a complex pattern of loss, gain and translocation.
PG  - 5693-706
AB  - The short arm of chromosome 8, 8p, is often rearranged in carcinomas, typically 
      showing distal loss by unbalanced translocation. We analysed 8p rearrangements in 
      48 breast, pancreatic and colon cancer cell lines by fluorescence in situ 
      hybridization (FISH) and array comparative genomic hybridization, with a tiling 
      path of 0.2 Mb resolution over 8p12 and 1 Mb resolution over chromosome 8. 
      Selected breast lines (MDA-MB-134, MDA-MB-175, MDA-MB-361, T-47D and ZR-75-1) 
      were analysed further. Most cell lines showed loss of 8p distal to a break that 
      was between 31 Mb (5' to NRG1) and the centromere, but the translocations were 
      accompanied by variable amplifications, deletions and inversions proximal to this 
      break. The 8p12 translocation in T-47D was flanked by an inversion of 4 Mb, with 
      a 100 kb deletion at the proximal end. The dicentric t(8;11) in ZR-75-1 carries 
      multiple rearrangements including interstitial deletions, a triplicated 
      translocation junction between NRG1 and a fragment of 11q (unconnected to CCND1), 
      and two separate amplifications, of FGFR1 and CCND1 . We conclude that if there 
      is a tumour suppressor gene on 8p it may be near 31 Mb, for example WRN; but the 
      complexity of 8p rearrangements suggests that they target various genes proximal 
      to 31 Mb including NRG1 and the amplicon centred around ZNF703/FLJ14299.
FAU - Pole, J C M
AU  - Pole JC
AD  - Cancer Genomics Program, Department of Pathology, University of Cambridge, 
      Hutchison/MRC Research Centre, Cambridge, UK.
FAU - Courtay-Cahen, C
AU  - Courtay-Cahen C
FAU - Garcia, M J
AU  - Garcia MJ
FAU - Blood, K A
AU  - Blood KA
FAU - Cooke, S L
AU  - Cooke SL
FAU - Alsop, A E
AU  - Alsop AE
FAU - Tse, D M L
AU  - Tse DM
FAU - Caldas, C
AU  - Caldas C
FAU - Edwards, P A W
AU  - Edwards PA
LA  - eng
GR  - 2005NOV68/BCN_/Breast Cancer Now/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060424
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Cell Line, Tumor
MH  - *Chromosome Aberrations
MH  - Chromosomes, Artificial, Bacterial
MH  - *Chromosomes, Human, Pair 8
MH  - Colonic Neoplasms/*genetics
MH  - Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Hybridization
MH  - Pancreatic Neoplasms/*genetics
EDAT- 2006/04/26 09:00
MHDA- 2006/10/19 09:00
CRDT- 2006/04/26 09:00
PHST- 2006/04/26 09:00 [pubmed]
PHST- 2006/10/19 09:00 [medline]
PHST- 2006/04/26 09:00 [entrez]
AID - 1209570 [pii]
AID - 10.1038/sj.onc.1209570 [doi]
PST - ppublish
SO  - Oncogene. 2006 Sep 14;25(41):5693-706. doi: 10.1038/sj.onc.1209570. Epub 2006 Apr 
      24.