PMID- 16637889
OWN - NLM
STAT- MEDLINE
DCOM- 20060731
LR  - 20150813
IS  - 1398-9219 (Print)
IS  - 1398-9219 (Linking)
VI  - 7
IP  - 6
DP  - 2006 Jun
TI  - The high-affinity immunoglobulin-E receptor (FcepsilonRI) is endocytosed by an 
      AP-2/clathrin-independent, dynamin-dependent mechanism.
PG  - 673-85
AB  - Aggregation of the high-affinity immunoglobulin E (IgE) receptor (FcepsilonRI), 
      expressed on mast cells and basophils, initiates the immediate hypersensitivity 
      reaction. Aggregated FcepsilonRI has been reported to rapidly migrate to lipid 
      rafts in RBL-2H3 cells. We confirmed that aggregated FcepsilonRI is found in the 
      lipid raft fractions of cellular lysates. Furthermore, we show that the 
      cross-linked FcepsilonRI remains associated with detergent-resistant structures 
      upon internalization. Previous morphological studies have reported that 
      aggregated FepsiloncRI is endocytosed via clathrin-coated pits, which in general 
      are not lipid raft associated. To address this apparent discrepancy, we employed 
      siRNA to suppress expression of components of the clathrin-mediated 
      internalization machinery, namely, clathrin heavy chain, and the AP-2 
      (alpha-adaptin or mu2-subunit). Transferrin receptor (TfR) is endocytosed by a 
      clathrin-mediated process and, as expected, each transfected siRNA caused a two 
      to threefold elevation of TfR surface expression and almost completely inhibited 
      its endocytosis. In contrast, there was no effect on surface expression levels of 
      FcepsilonRI nor on the endocytosis of the dinitrophenyl-human serum albumin 
      (DNP-HSA)/IgE/FcepsilonRI complex. On the contrary, internalization of 
      DNP-HSA/IgE/FcepsilonRI was inhibited by overexpression of a dominant-negative 
      dynamin mutant. We conclude that internalization of cross-linked FcRI does not 
      require the AP-2/clathrin complex but is dynamin-dependent and may be lipid raft 
      mediated.
FAU - Fattakhova, Gul'nar
AU  - Fattakhova G
AD  - Receptor Cell Biology Section, Laboratory of Allergic Diseases, National 
      Institute of Allergy and Infectious Diseases, National Institutes of Health, 
      Rockville, MD 20852, USA.
FAU - Masilamani, Madhan
AU  - Masilamani M
FAU - Borrego, Francisco
AU  - Borrego F
FAU - Gilfillan, Alasdair M
AU  - Gilfillan AM
FAU - Metcalfe, Dean D
AU  - Metcalfe DD
FAU - Coligan, John E
AU  - Coligan JE
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Transferrin)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factor AP-2)
RN  - 114899-12-6 (Clathrin Heavy Chains)
RN  - EC 3.6.5.5 (Dynamins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Clathrin Heavy Chains/antagonists & inhibitors/genetics/*metabolism
MH  - Cross-Linking Reagents
MH  - Dynamins/genetics/*metabolism
MH  - Endocytosis
MH  - Membrane Microdomains/metabolism
MH  - Mutation
MH  - RNA, Small Interfering/genetics
MH  - Rats
MH  - Receptors, IgE/*metabolism
MH  - Receptors, Transferrin/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Transcription Factor AP-2/antagonists & inhibitors/genetics/*metabolism
MH  - Transfection
EDAT- 2006/04/28 09:00
MHDA- 2006/08/01 09:00
CRDT- 2006/04/28 09:00
PHST- 2006/04/28 09:00 [pubmed]
PHST- 2006/08/01 09:00 [medline]
PHST- 2006/04/28 09:00 [entrez]
AID - TRA423 [pii]
AID - 10.1111/j.1600-0854.2006.00423.x [doi]
PST - ppublish
SO  - Traffic. 2006 Jun;7(6):673-85. doi: 10.1111/j.1600-0854.2006.00423.x.