PMID- 16638207
OWN - NLM
STAT- MEDLINE
DCOM- 20071214
LR  - 20181201
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 14
IP  - 2
DP  - 2006 Apr
TI  - [Origin of mesenchymal stem cells in bone marrow of patients after allogeneic 
      stem cell transplantation].
PG  - 322-6
AB  - The aim of this study was to investigate the origin of bone marrow-derived 
      mesenchymal cells in 34 patients who had received a sex-mismatched hematopoietic 
      stem cells transplant (HSCT). The mesenchymal stem cells (MSC) from 34 patients 
      were collected for test. The different passage MSC of patients were analyzed by 
      flow cytometry (FCM) to reveal the cell surface antigen expression. The 
      polymerase chain reaction (PCR) analysis of the amelogenin (AMEL) genes was used 
      to detect donor cells from host cells. The cultured MSC were stained by 
      fluorescence in situ hybridization (FISH) probes for chromosomes X 
      (Xp11.1-Xq11.1) and Y (Yq12) to distinguish donor cells from host cells. The 
      results indicated that 31 out of 34 cases showed the confluent stroma and 24 out 
      of these 31 cases were successfully passaged for more than 5 generations which 
      could be used for PCR and FISH analysis. According to FCM results the number of 
      CD14+CD45+ cells, which was regarded as monocyte/macrophage from the cultured MSC 
      (passage 5) was less than 0.04%. In PCR assay, the marrow-derived MSC from the 
      passage 5 were found to be of host origin. FISH assay demonstrated that 
      marrow-derived MSC from the passage 5 were found to be of host origin up to 100%. 
      It is concluded that the origin of MSC in bone marrow of patients after 
      allogeneic stem cell transplantation was confirmed to be derived still from the 
      recipients their own.
FAU - Wang, Jing
AU  - Wang J
AD  - People's Hospital, Institute of Hematology, Peking University, Beijing 100044, 
      China.
FAU - Liu, Kai-Yan
AU  - Liu KY
FAU - Lu, Dao-Pei
AU  - Lu DP
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Lipopolysaccharide Receptors)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Bone Marrow Cells/pathology
MH  - Cells, Cultured
MH  - Chromosomes, Human, X/genetics
MH  - Chromosomes, Human, Y/genetics
MH  - Female
MH  - Hematologic Neoplasms/therapy
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukocyte Common Antigens/analysis
MH  - Lipopolysaccharide Receptors/analysis
MH  - Male
MH  - Mesenchymal Stem Cells/*pathology
MH  - Middle Aged
MH  - *Peripheral Blood Stem Cell Transplantation
MH  - Transplantation Conditioning
MH  - Transplantation, Homologous
EDAT- 2006/04/28 09:00
MHDA- 2007/12/15 09:00
CRDT- 2006/04/28 09:00
PHST- 2006/04/28 09:00 [pubmed]
PHST- 2007/12/15 09:00 [medline]
PHST- 2006/04/28 09:00 [entrez]
AID - 1009-2137(2006)02-0322-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Apr;14(2):322-6.