PMID- 16638301
OWN - NLM
STAT- MEDLINE
DCOM- 20100907
LR  - 20121115
IS  - 1001-0939 (Print)
IS  - 1001-0939 (Linking)
VI  - 29
IP  - 1
DP  - 2006 Jan
TI  - [Effects of sense vascular endothelial growth factor cDNA transfection on 
      mononuclear chemotaxis protein-1 expression in rat pulmonary microvessel 
      endothelial cells].
PG  - 44-7
AB  - OBJECTIVE: To study the transformation characteristics of tight junction of 
      microvessel endothelial cells in bleomycin (BLM) induced pulmonary fibrosis (PF), 
      and the effects of vascular endothelial growth factor (VEGF) on mononuclear 
      chemotaxis protein-1 (MCP-1) mRNA expression in pulmonary microvessel endothelial 
      cells (EC). METHODS: Forty healthy rats were equally divided into a control and 
      an experimental group in random. In the experimental group, PF were induced by 
      BLM application. In each group, 10 rats were instilled by lanthanum sal at 3, 7, 
      14 and 28 d after BLM application, and lung samples were made and observed by 
      transmission electron microscopy. The pulmonary microvessel EC of the other 10 
      rats in each group were preserved by tissue culture methods at the same time, and 
      the cells were transfected with sense VEGF cDNA, and then MCP-1 mRNA expression 
      was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) 
      technique. RESULTS: Blood vessel endothelial cells of the control group were 
      intact. The basement membrane was shown as a continuous line. Granules of 
      lanthanum sal did not cross the tight junction of endothelial cells. The width of 
      the junction gap in rats of the experimental group treated at different times was 
      increased and lanthanum particles of high density were seen in the gap junction, 
      particularly on day 3, and were distributed in the area of subendothelium. The 
      MCP-1 mRNA expression in VEGF transfected microvessel EC was significantly higher 
      than that of the control group (1.21 +/- 0.22 vs 0.36 +/- 0.06, P < 0.05). 
      CONCLUSION: In BLM induced PF, the opening of the tight junction of EC, and the 
      high expression of MCP-1 induce inflammatory cell infiltration and cytokine 
      over-secretion, which in turn enhances proliferation of fibroblasts, one of the 
      important factors underlying lung fibrosis.
FAU - Cui, Guang-bin
AU  - Cui GB
AD  - Department of Radiology, Tangdu Hospital, Fourth Military Medical University, 
      Xi'an 710038, China.
FAU - Wei, Jing-guo
AU  - Wei JG
FAU - Wang, Wei
AU  - Wang W
FAU - Wei, Long-xiao
AU  - Wei LX
FAU - Yin, Qian
AU  - Yin Q
FAU - Yang, Hao
AU  - Yang H
FAU - Huang, Xiao-feng
AU  - Huang XF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Jie He He Hu Xi Za Zhi
JT  - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal 
      of tuberculosis and respiratory diseases
JID - 8712226
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/genetics/*metabolism
MH  - DNA, Complementary/genetics
MH  - Endothelial Cells/*metabolism
MH  - Genetic Therapy
MH  - Pulmonary Fibrosis/*metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tight Junctions/metabolism
MH  - *Transfection
MH  - Vascular Endothelial Growth Factor A/*genetics
EDAT- 2006/04/28 09:00
MHDA- 2010/09/08 06:00
CRDT- 2006/04/28 09:00
PHST- 2006/04/28 09:00 [pubmed]
PHST- 2010/09/08 06:00 [medline]
PHST- 2006/04/28 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Jie He He Hu Xi Za Zhi. 2006 Jan;29(1):44-7.