PMID- 16640885
OWN - NLM
STAT- MEDLINE
DCOM- 20080214
LR  - 20131121
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 41
IP  - 3
DP  - 2006 Mar
TI  - [Oocyte activation with calcium ionophore A23187 and puromycin on human oocytes 
      that fail to fertilize after intracytoplasmic sperm injection].
PG  - 182-5
AB  - OBJECTIVE: To investigate the effect of assisted oocyte activation with calcium 
      ionophore A23187 and puromycin on human oocytes that fail to fertilize after 
      intracytoplasmic sperm injection (ICSI). METHODS: All 113 discarded oocytes that 
      showed no evidence of fertilization at 16 - 18 hours after in vitro maturation 
      (IVM)-ICSI cycles and conventional ICSI were assigned to four groups according to 
      the time after ICSI: IVM-ICSI 22-hour group (n = 33), IVM-ICSI 44-hour group (n = 
      18), ICSI 44-hour group (n = 37) and ICSI 68-hour group (n = 25). All 
      unfertilized oocytes were exposed to calcium ionophore A23187 (5 micromol/L) for 
      5 minutes and subsequently incubated with puromycin (10 microg/ml) for 4 hours. 
      After incubation, the oocytes were cultured in vitro for 3 - 5 days. The 
      activation rate, proportion of oocytes that showed pronucleus formation and 
      cleavage rate were calculated after activation. Sex chromosomal analysis was 
      performed by dual color fluorescence in situ hybridization (FISH) on the embryos 
      that displayed two pronuclei and a second polar body. RESULTS: The combination of 
      calcium ionophore A23187 with puromycin could activate the unfertilized oocytes 
      22 - 68 hours after ICSI. Best results were achieved in IVM-ICSI 22-hour group, 
      which elicited 88% (29/33) of activation rate, 62% (18/29) of cleavage rate and 
      28% (5/18) of 4-cell embryos. One embryo in this group developed to the morular 
      stage. The activation rate and developmental potential of the activated embryos 
      in IVM-ICSI 44-hour group, ICSI 44-hour group and ICSI 68-hour group decreased. 
      FISH analysis showed 4 embryos with XX and 9 embryos with XY in 16 embryos. 
      CONCLUSIONS: The combination of calcium ionophore A23187 with puromycin could 
      effectively activate unfertilized oocytes 22 - 68 hours after ICSI. The cultured 
      time of unfertilized oocytes after ICSI affects activation efficiency and 
      developmental potential of the activated embryos. The activated zygotes that 
      display two pronuclei and a second polar body can develop normally.
FAU - Lu, Qun
AU  - Lu Q
AD  - Reproductive Medicine Center, Shandong Provincial Hospital, Shandong University, 
      Jinan 250021, China.
FAU - Chen, Zi-jiang
AU  - Chen ZJ
FAU - Gao, Xuan
AU  - Gao X
FAU - Ma, Shui-ying
AU  - Ma SY
FAU - Li, Mei
AU  - Li M
FAU - Hu, Jing-mei
AU  - Hu JM
FAU - Li, Yuan
AU  - Li Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (Ionophores)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 4A6ZS6Q2CL (Puromycin)
SB  - IM
MH  - Calcimycin/*pharmacology
MH  - Cells, Cultured
MH  - Cleavage Stage, Ovum/drug effects
MH  - Embryo, Mammalian/cytology/drug effects
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Ionophores/pharmacology
MH  - Karyotyping
MH  - Male
MH  - Oocytes/cytology/*drug effects/metabolism
MH  - Puromycin/*pharmacology
MH  - *Sperm Injections, Intracytoplasmic
MH  - Time Factors
MH  - Zygote/cytology/drug effects/growth & development
EDAT- 2006/04/28 09:00
MHDA- 2008/02/15 09:00
CRDT- 2006/04/28 09:00
PHST- 2006/04/28 09:00 [pubmed]
PHST- 2008/02/15 09:00 [medline]
PHST- 2006/04/28 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2006 Mar;41(3):182-5.